What step normally associated with staining bacterial cells is omitted when the dimensions of cells are determined? Why?

Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…

A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…

Q: Define fluorescent staining.

A: The advent of microscopy started with the development of optical microscopy. Then the level of…

Q: Why is heat necessary in spore staining?

A: BASIC INFORMATION  SPORULATION  It is a process during which spores are formed.  We can find it in…

Q: What particular features of fluorescence make it useful for microscopy?

A: Step 1 Microscopes are an instrument which is used for observing small objects and finer internal…

Q: What is gram staining and its purpose? Why is it only applicable in bacteria?

A: Introduction: Differential staining methods like Gram staining, acid-fast staining, endospore…

Q: hich Is The More Important Step In Gram Stain?

A: Hans Christian Gram invented the Gram stain in 1884. (Gram,1884).

Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…

A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…

Q: Basic stains are more effective for bacterial staining than acidic dyes. Why? Which component on the…

A: Answer 1)Basic stains , which carries the postive charge chromogen are preferred because the…

Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?

A: bacteria like  Mycobacterium and Nocardia and the apicomplexan Cryptosporidium are not stained…

Q: In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?

A: Gel-filtration chromatography is a type of partition chromatography that is used to separate…

Q: Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed…

A: Bacteria have cell walls made up of polysaccharides that give them strength.Bacterial cell walls…

Q: What is the difference between simple stain and complex stain? Describe the color of an S. aureus…

A: Most types of cells don't have a normal pigment and are in this way challenging to see under the…

Q: If you were working with an unlabeled simple stained smear, would you be able to identify the…

A: Staining is used to colour biological specimen such as cell to identify them. Simple staining…

Q: In four steps, describe the simple staining procedure.

A: A stain is used to clearly view a specimen under the microscope. In simple staining, bacteria are…

Q: Which bacterial morphology is demonstrated by this simple stain? choose as many as are correct

A: For all types of bacterial cells, a simple staining method may be used to give contrast to the…

Q: Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can…

A: According to the guidelines we have to answer first 2-3 question rest you can ask separately thank…

Q: What stain would you use to differentiate between bean and ant cells? Explain and include your…

A: Staining is a procedure that involves the application of colored chemicals called dyes to specimens.…

Q: why does sometimes acid-fast bacteria accept the spore stain and spores accept the acid-fast stain.

A: Acid-fast staining technique is a differential staining technique. The bacteria which get stained by…

Q: What is the purpose of using a biological stain when microscopically examining cellular components?

A: Staining is a technique that is adopted to enhance the visibility of the specimens under study that…

Q: Why is it important that the agarose is exposed to ethidium bromide, which is either added to the…

A: Ethidium bromide is utilized during separation of fragments of DNA by agarose gel electrophoresis.…

Q: plain Why do acidic dyes not stain bacterial cells?

A: Staining is the process of changing the colour of a substance to make it easier to examine tissues,…

Q: Why is nigrosin-water- bacteria puddle need to be mixed in a Simple/Negative staining

A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…

Q: list the major steps, in sequence needed to perform the schaeffer-fulton and crystal violet…

A: Endospores are structures produced within certain bacterial cells that allow them to survive harsh…

Q: What's the theory and conclusion of a acid fast stain?

A: It was Ziehl who originally devised differential staining procedures, that were later refined by…

Q: a. Why must heat be used with the application of the primary stain during endospore staining?

A: Defination-  An endospore is a non -reproductive structure  which is dormant and tough produced by…

Q: What is the secondary stain in both acid-fast stain methods?

A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…

Q: Explain the function of stacking gel, separating gel, and Coomassie blue stain in the…

A: Answer : Stacking Gel : The purpose of the stacking gel packing is to combine all the samples of the…

Q: Use the table to differentiate between agarose gel electrophoresis and SDS-PAGE based on the…

A: Agarose Gel Electrophoresis is the technique of separating DNA or protein. SDS-PAGE is the technique…

Q: About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this…

A: Gram staining technique is a differential test based on the composition of bacteria cell wall. It…

Q: why the Gram stain can differentiate between Gram positive and Gramnegative cells?

A: *Gram staining is a technique used to differentiate two groups of bacteria into gram positive and…

Q: Why don't you heatfix a negative stain slide?

A: Negative staining is used to investigate the physical structure, size, and organization of…

Q: What is the advantage of phase-contrast microscopyover bright-field microscopy?

A: Phase-contrast microscopy is an optical microscopy technique that converts phase shifts in light…

Q: What major advantage does phase-contrast microscopy haveover staining?

A: Phase-contrast microscopy is an optical microscopy technique used in cells. When the light passes…

Q: List the step-by-step procedure of Gram staining.

A: Gram staining, established by Danish Microbiologist Hans Christian Gram in 1884, is by far the most…

Q: Define dyes and describe the basic chemistry behind the processof staining.

A: Detection and observation of cells and microorganisms in their natural state under microscope is…

Q: In Gram staining method, list the reagents and state their role for each step of the procedure? In…

A: Bacteria are unicellular, prokaryotic organicism which are devoid of membrane bound organelles and a…

Q: ou make a smear of E. coli and then simple stain the slide with methylene blue. If you failed to use…

A: The Gram stain is a differential staining method used for classifying bacteria into either…

Q: Why is it incorrect to say that the GFP is a “staining” method?

A: Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that…

Q: What macromolecules does DAPI stain? What are its advantages and disadvantages?

A: Genetics is the branch of biology that deals with genetic material like DNA, RNA, inheritance.…

Q: bacteria require staining before microscopic examination?

A: Bacteria are single-celled organisms with a small size. Bacteria can be found practically everywhere…

Q: Why is the resolution so superior in the electron microscope?

A: A beam of electrons that travel in wavelike patterns when accelerated to high speeds form the images…

Q: what would be the medical significance of finding a positive endospore stain result from bacilli…

A: Endospores are thick walled structures found in the genus Bacillus and Clostridium.

Q: The DNA fragments separated on an agarose gel can be visualised after staining with what?

A: Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis…

Q: For What purpose  is Giemsa stain used for?

A: Staining is a technique used in microscopy. Staining is mainly used in biology to highlight the…

Q: In acid fast staining, lipoidal material of acid fast cells absorbs the methylene blue. Upon the…

A:

Q: What type of chromophore is associated with a negative stain?

A: Bacteria are unicellular prokaryotic organisms that maintain a definite shape since they possess the…

Q: What is the principle of image formation on transmission election microscope and scanning electron…

A: The general biological microscope helps in the visualisation of the image of a particular sample…

Q: Why would specimens viewed with a compound microscope be thin and/or chemically cleared?

A: The human eye can see objects upto 0.1 millimeters. If the objects are smaller than this, the human…

Q: Name two fixatives used in staining?

A: Histology is the study of ultrastructure of tissues using thin sectioning techniques like microtomy,…

Q: How do stains used for light microscopy compare withthose used for electron microscopy?

A: The micro-organism is the organism that is too small to be seen with the naked eye. It is viewed by…

Bacterial Morphology

The bacteria are prokaryotic organisms that are single-celled, and are found to exist as free-living and possess a microscopic size. The morphology is found to vary in the bacteria, where some of them are identified as individual organisms and the others are detected as colonies. The size and shape of the bacterial cell also represent its morphology. 

Bacterial cell structure

Bacteria are single-celled, tiny creatures that may enter healthy tissues and grow rapidly. Bacteria are microscopic organisms that are tiny and unicellular. These are members of the prokaryote kingdom. They live in water, air, soil, and all-natural environments. They are used in industrial and therapeutic processes, and they support a wide range of plant and animal life. The first organism to appear on the planet. Bacteria-like creatures are the oldest known fossils. Bacteria can consume a wide range of organic and inorganic elements, and some may even survive in harsh conditions.