When does the trp operon allow its gene to be read? * when tryptophan is not present when lactose is present Owhen lactose is not present O when tryptophan is present What is the importance of lactase? * Lactase helps regulate when a gene is read. Lactase in not important to the cell. Lactase breaks down lactose sugar molecules for the cell. Lactase helps to regulate when proteins are produced in the cell.

When does the trp operon allow its gene to be read? * when tryptophan is not present when lactose is present Owhen lactose is not present O when tryptophan is present What is the importance of lactase? * Lactase helps regulate when a gene is read. Lactase in not important to the cell. Lactase breaks down lactose sugar molecules for the cell. Lactase helps to regulate when proteins are produced in the cell.

Name: When does the trp operon allow its gene to be read? *when tryptophan
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Description: When does the trp operon allow its gene to be read? *when tryptophan is not presentwhen lactose is presentwhen lactose is not presentwhen tryptophan is presentWhat is the importance of lactase? *Lactase helps regulate when a gene is read.OLactase in

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 2TYU

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Study the illustration of the lac operon below. Normally, the genes are switched off when lactose is not present. Lactose activates the genes, which code for enzymes that allow the cell to use lactose. Mutations can alter the functions of this operon. Predict how the following mutations would affect the function of the operon the absence and presence of lactose. Mutation of regulatory gene; repressor will not bind to lactose. Mutation of operator; repressor will not bind to operator. Mutation of regulatory gene; repressor will not bind to operator. Mutation of promoter; RNAP will not attach to promoter.
Study the depiction of the lac operon in Figure 11.2. Normally, the genes are turned off when lactose is not present. Lactose activates the genes, which code for enzymes that enable the cell to use lactose. Mutations can alter the function of this operon. Predict how the following mutations would affect the function of the operon in the presence and absence of lactose: a. mutation of regulatory gene; repressor will not bind to lactose b. mutation of operator; repressor will not bind to operator c. mutation of regulatory gene; repressor will not bind to operator d. mutation of promoter; RNA polymerase will not attach to promoter
A mutation at the operator prevents the regulator protein from bindingWhat effect will this mutation have in the following types of operons? a. Regulator protein is a repressor of a repressible operon. b. Regulator protein is a repressor of an inducible operon.
In the lac operon (above), what happens when glucose levels are low, but lactose levels increase? the repressor protein is detached from the operator, and transcription is not initiated the repressor protein is attached to the operator, and transcription is not initiated the repressor protein is detached from the operator, and transcription is initiated the repressor protein is attached to the operator, and transcription is initiated. Check all of the above occur at equal rates when glucose levels are low but lactose levels are high
The lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose metabolism. Mutations to various regions of the lac operon have been discovered which affect both the control of gene expression and the function of the gene products. These mutations include: I - = produces a repressor protein that cannot bind to the operator region IS = produces a repressor protein that cannot be removed from the operator region OC = cannot bind a repressor protein Z - = produces a defective (non-functioning) β-galactosidase enzyme + = wild type Detail the functionality of the lac operon under the following genetic and cellular conditions. You do not need to consider the role of CAP in your analysis. Genes Repressed (yes/no) Constitutive (yes/no) IS O+ Z+ IS OC Z+
The lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose metabolism. Mutations to various regions of the lac operon have been discovered which affect both the control of gene expression and the function of the gene products. These mutations include: I - = produces a repressor protein that cannot bind to the operator region IS = produces a repressor protein that cannot be removed from the operator region OC = cannot bind a repressor protein Z - = produces a defective (non-functioning) β-galactosidase enzyme + = wild type Detail the functionality of the lac operon under the following genetic and cellular conditions. You do not need to consider the role of CAP in your analysis. Genes Repressed (yes/no) Constitutive (yes/no) I+ OC Z+ I+ O+ Z+ (Lactose present) I- O+ Z+
A mutation in the repressor LacI causes it to lose the ability to bind its effector, allolactose. What will be the effect of this mutation in the presence of allolactose (assume glucose is absent)? a) LacI cannot bind to the lac operator (lacO) and transcription of the lac operon is blockedb) LacI is permanently bound to the lac operator (lacO) and transcription of the lac operon is blocked c) LacI cannot bind to the lac operator (lacO) and transcription of the lac operon increasesd) LacI is permanently bound to the lac operator (lacO) and transcription of the lac operon increases
Mutations may have an effect on the expression of the lac operon and the trp operon. Would the following mutations have a cis- or transeffect on the expression of the protein-encoding genes in the operon? A. A mutation in the operator site that prevents lac repressor from binding to it B. A mutation in the lacI gene that prevents lac repressor from binding to DNA C. A mutation in the trpL gene that prevents attenuation
What is a specific region in the trp operon that you could mutate so it expresses the trp operon in conditions of excess tryptophan. Why will this mutation cause this? Operon structure: promoter, operater, structural genes promoter - DNA region that is the site of RNA polymerase sigma binding and transcription initiation operator – DNA region that influences gene expression through its interaction with a protein (transcription factor) structural genes – gene whose expression is being regulated; encodes a polypeptide or functional RNA
Which of the following is true about the Lac operon? Group of answer choices An operon with a constitutive operator (O^C mutation) will transcribe in the presence of a super repressor It is a positive repressible operon that is influenced mainly by fluctuations in glucose levels Produces a large quantity of tryptophan in order to synthesize lactase It is found in eukaryotes and is responsible for creating an enzyme that breaks down lactose
How does tryptophan regulates its own production? a. When tryptophan levels are high, it binds to the trp repressor, which changes its shape and binds to the operator sequence in the trp operon. b. When tryptophan levels are high, it binds to the trp repressor, which changes its shape and binds to the promoter sequence in the trp operon. c. When tryptophan levels are high, it binds to the trp repressor, which changes its shape and falls off from the operator sequence in the trp operon. d. When tryptophan levels are high, it binds to the trp repressor, which changes its shape and falls off from the promoter sequence in the trp operon.
A mutation in the operator region of the trp operon can prevent the trp repressor from binding to this operator. When these mutant cells are placed in a solution containing chicken broth, which of the following happens? a. Tryptophan would bind to the repressor. b. The Repressor would bind to the operator irrespective of the presence of amino acids in the environment. c. The transcription of the trip operon would be inhibited. d. The transcription of the repressor protein is inhibited