When Meselson and Stahl grew E. coli in 15N mediumfor many generations and then transferred the cells to 14Nmedium for one generation, they found that the bacterial DNA banded at a density intermediate between that ofpure 15N DNA and pure 14N DNA following equilibrium density centrifugation. When they allowed thebacteria to replicate one additional time in 14N medium, they observed that half of the DNA remained atthe intermediate density, while the other half banded atthe density of pure 14N DNA. What would they haveseen after an additional generation of growth in 14Nmedium? After two additional generations?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
When Meselson and Stahl grew E. coli in 15N medium
for many generations and then transferred the cells to 14N
medium for one generation, they found that the bacterial DNA banded at a density intermediate between that of
pure 15N DNA and pure 14N DNA following equilibrium density centrifugation. When they allowed the
bacteria to replicate one additional time in 14N medium, they observed that half of the DNA remained at
the intermediate density, while the other half banded at
the density of pure 14N DNA. What would they have
seen after an additional generation of growth in 14N
medium? After two additional generations?
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