Question
Asked Oct 6, 2019
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when pure hemoglobin (molecular mass of 64,000Da)is separated by the following methods ,what major molecular mass species do you expect to see? explain with one sentence.

gel filteration :

gel filtration with 6M urea:

SDS-PAGE with DTT:

SDS-PAGE:

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Expert Answer

Hemoglobin is a tetramer made up of four subunits, namely, two alpha and two beta subunits. The molecular weight of the alpha and beta subunits are 15.1 kDa and 15.8 ...

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