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When testing the sensitivity of antibiotics using the Kirby-Bauer method, the Petri Dish with Escherichia coli had the following results:
Antibiotic Zone of inhibition
Penicillin 0 mm
Ampicillin 9 mm
Ciproflaxacin 30 mm
Tetracycline 31 mm
What are the best conclusions you can make from the choices below?
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- Briefly explain how you could use the color of the Coomassie assay sample solution to determine if anunknown sample needs to be diluted in order to measure the sample’s concentrationI missed a couple of my labs due to covid and am struggling to teach myself how to do serial dilution, dilution, dilution factors, and colony forming units per ml of original stock. I thought I was understanding everything until it was time for me to take the practice quiz and I realized that I understood nothing.You are testing a potential new antibiotic for its effectiveness at killing Streptococcus pneumoniae, the bacterium that causes pneumonia. As a first test of this drug, you grow the bacteria in the presence of different concentrations of this drug and analyze its effect on the growth of the bacteria. The data are shown in the table below listing the drug concentration in milliMolar (mM) against the percentage of growth relative to the control (no drug). Construct an appropriate graph for the presentation of these data. Drug Concentration (mM) Percentage of Growth (%) 0 100 1 83 2 76 3 62 4 55 5 30 6 15 7 2
- Explain the effects of ampicillin and X-gal in the following 4 different LB media plates LB LB + X-gal LB + Amp LB + Amp + X-galOrder: streptomycin 500 mg IM daily for 8 days. If you have vials of this antibiotic that are labeled as 1 g/2.5 mL, how many milliliters would you administer?Enumerate and describe at least three (3) big scale pharmaceutical methods on reducing particle size.
- List 3 advantages and 3 disadvantages of the protein assays Biuret, Folin (Lowry), and Bradford.What does the zone of inhibition imply? Does the measurement of the zone ofinhibition imply that one antibiotic is better than the other? Support your answer.State the principle that underlies the following biochemical tests: a. Voges-Proskauer test
- Why is it a good idea to perform three trials at each temperature? How would you modify the experiment to produce more reliable results?100ml of LB media with 25 μg/ml of Amp and 100 μg/ml of Kan final concentration. You have 100ml of LB provided and Amp and Kan stocks at 100 mg/ml and 50 mg/ml provided. Determine how much of each antibiotic stock solution you need to add to 100ml of LB to reach desired antibiotic concentration.