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A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…
Q: What is the color results of tyrosine,phenylalanine,alanine in millon test?
A: Million's test is a protein qualitative test: Principle: Million's test detect tyrosine as its the…
Q: Why safranin is used as the sample dye?
A: Safranin is utilized as a counterstain in some staining conventions, shading cell cores red. This is…
Q: Explain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosin
A: The living cells can be seen under a microscope. The cells is not visible properly as it is…
Q: What will happen if the protein that you are assaying using zymography is not fixed properly using…
A: Zymogram analysis is done in order to and characterize collagenases, metalloproteinases, and other…
Q: Explain why it is important to use only a small amount of bacteria when preparing a smear. What are…
A: Many laboratory techniques, including the Gram-stain, involve the creation of a smear. The goal of…
Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?
A: bacteria like Mycobacterium and Nocardia and the apicomplexan Cryptosporidium are not stained…
Q: What are the principle and basic concepts of DIFFERENTIAL staining?
A: The simple dye is used in the technique of simple staining to highlight only the specific structures…
Q: Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain…
A: In capsule staining procedure “we do not heat fix and rinse the smear with water” as heat and water…
Q: What is the difference between simple stain and complex stain? Describe the color of an S. aureus…
A: Most types of cells don't have a normal pigment and are in this way challenging to see under the…
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A: Pasteurization is a process that helps in keeping the milk fresh and helps in avoiding the…
Q: In four steps, describe the simple staining procedure.
A: A stain is used to clearly view a specimen under the microscope. In simple staining, bacteria are…
Q: what are the regeants and results for Mann’s methyl blue-eosin stain
A: Mann's methyl blue eosin strain- is formulated to demonstrate Negri bodies in the tissue section it…
Q: What is a differential stain? why is it used?
A: Differential stain is a type of stain use to identify organisms.
Q: What is the principle of Giemsa staining and components of Giemsa stain?
A: The cells are the basic structural and fundamental unit of the living system. It consists of…
Q: Write the procedure for celloidin embedding.
A: Hi! Thanks for your question. But as you have posted multiple questions, I am answering the first…
Q: What are the principle and basic concepts of NEGATIVE staining?
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: What is the color result of 1% solution of casein, Albumin, Gelatin in ninhydrin test? Include…
A: Ninhydrin test: Chemical test to detect presence of ammonia, primary/secondary amines, or amino…
Q: Describe the appearance of the protein solutions in each of the tubes after addition of the…
A: Proteins are complex nitrogen-containing organic molecules that involve all aspects of the live…
Q: Explain the principle of capsule staining?
A: The staining process is performed for visualisation purpose under microscope. Actually the chemical…
Q: What is the procedure of beta method?
A: Beta-lactamase test is used for the detection of enzyme beta-lactamase. This enzyme provides the…
Q: The capsule stain is considered a negative stain
A: Staining is a technique in microbiology so as to enhance the view by contrasting it. Stains are…
Q: Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct) a…
A: Simple stains- A stain that employs a single stain to color a bacteria: they are quick and easy.…
Q: What other method could be used to purify lysozyme from white using its unusual pl?
A: The unique charge properties of lysozyme with a very high pI of about 10.5 can be used in the…
Q: What are the Types of Differential Stains? explain with an example.
A: Bacteria are prokaryotes that are devoid of any membrane-bound organelles. They are differentiated…
Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
Q: What is the advantage of the Kinyoun staining procedure over the Ziehl-Neelsen method?
A: Kinyoun staining is a technique used to stain bacteria. Specifically, acid-fast species of the…
Q: What step normally associated with staining bacterial cells is omitted when the dimensions of cells…
A: Step 1 Gram staining is a technique of staining that is used to differentiate and categorize…
Q: Why is it necessary to perform a negative stain to visualize capsules?
A: Introduction: Negative staining: This uses an acidic stain. The results yield a clear cell with a…
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A: Honey is a naturally occurring sweetener that is originally derived from flower nectar and fruits.…
Q: a.) Give the purpose of heat fixation. b.) What can be observed in wet mounts or hanging-drop slides…
A: The Purpose of fixation of a smear is as follows: 1) Tissue Killing- As this will help to prevent…
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A: Osmolarity is defined as the number of moles of solute present in 1 litre of solution. When a…
Q: When using the blue/ white colony screening method, what do blue colonies represent ?
A: Blue- white screening is used for the identification of recombinant bacteria.
Q: About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this…
A: Gram staining technique is a differential test based on the composition of bacteria cell wall. It…
Q: Staining bacterial capsule can not be accomplished by?
A: Since most standard stains cannot enter the capsule because it is so tightly packed, it is difficult…
Q: Why differential centrifugation can cause contamination in pellet?
A: Differential centrifugation is a typical process used in microbiology and cytology to separate…
Q: What is the secondary stain in the spore stain?
A: Endospores are the structures that are formed by bacteria during unfavorable conditions (lack of…
Q: What is the mordant in the spore stain?
A: A mordant is any substance, which is used as a fixative to bind the dye on the substance.
Q: What is the procedure for methylene blue stain on e.coli?
A: Methylene Blue is a cationic stain, which binds to negatively charged parts of the cells, such as…
Q: What is a crystal violet stain and its purpose?
A: Crystal violet is that the name given to the cluster of similar organic compounds that are used as…
Q: What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What…
A: In microbiology, the technique that is used to enhance the contrast in samples, at the microscopic…
Q: Why is it incorrect to say that the GFP is a “staining” method?
A: Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that…
Q: the chemicals used in the gram staining method?
A: The bacteriologist Hans Christian Gram was developed gram staining method in 1884. the Gram staining…
Q: Aside from H&E, what other staining methods are applicable in Golgi apparatus, mitochondria, and…
A: Answer :- Subsequent to trying different things with presenting cells to gold and mercury, Golgi…
Q: In acid fast staining, lipoidal material of acid fast cells absorbs the methylene blue. Upon the…
A:
Q: What type of chromophore is associated with a negative stain?
A: Bacteria are unicellular prokaryotic organisms that maintain a definite shape since they possess the…
Q: What staining method would you use to verify that the isolate is not producing survival structures…
A: Staining is the procedure that helps to visualize the minute organisms under a microscope using…
Q: Write the procedure for celloidin embedding
A: Answer: Celloidin = It is basically the semisolid solution of pyroxylin in ether or alcohol, which…
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- Explain why it is important to use only a small amount of bacteria when preparing a smear. What are the two things that are stained in a capsule stain? What is NOT stained in a capsule stain?About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this type of coloring?What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary stain in the Ziehl-Neelsen method?