Question
Asked Oct 8, 2019
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Which light source will provide you with greater resolution than green light, blue or red light? why?

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Expert Answer

Step 1

The resolution is defined as the smallest distance between two molecules or substances that can be differentiated by the microscope. Numerical aperture is linked to the angular aperture and refractive index of the medium. High numerical aperture value gives better resolution.

Step 2

Resolution is dependent on the numerical aperture of the lens. High numerical aperture gives better resolution. But various other factors like illumination, type of specimen, degree of aberration correction. Resolution is useful in the magnification of the specimen in greater detail. The wavelength of light determines its resolving ability.

Another factor that governs the resolution is the wavelength of light used. Higher wavelength like Shorter wavelength gives higher resolution and larger wavelength of light gives a lower resolution. The most effective resolution in optical microscopy is achieved in near-ultraviolet light. It is followed by blue light, green light and finally the red light.

Step 3

In the visible spectrum, the wavelength of blue light is 450-490 nm and the resolving power is 0.21-0.24 µm. The wavelength of green light is 560-590 nm and its resolving power is 0.29-0.32 µm and that of red light is 635-700 nm and its resolving power ...

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