Which of the follow are correct statements about microscope handling and care? 1. Always use TWO HANDS to carry the microscope. 2. Only use LENS PAPER to clean the lenses. Never wipe back and forth, but simply make a gentle swipe across the lens. 3. A soft cloth should be used to clean the lenses. 4. Start and finish the day’s work at the LOWEST MAGNIFICATION. 5. Start and finish the day’s work at the HIGHEST MAGNIFICATION 6. Always BEGIN examining a specimen with a LOW POWER objective. 7. Always BEGIN examining a specimen with a HIGHEST POWER objective 8. On the compound microscope, NEVER use the coarse adjustment knob with the high power or oil objective. 9. On the compound microscope, ALWAYS use the coarse adjustment knob with the high power or oil objective. 10. Unplug the microscope by pulling on the plug, not the cord.
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
Which of the follow are correct statements about microscope handling and care?
1. Always use TWO HANDS to carry the microscope.
2. Only use LENS PAPER to clean the lenses. Never wipe back and forth, but simply make a gentle swipe across the lens.
3. A soft cloth should be used to clean the lenses.
4. Start and finish the day’s work at the LOWEST MAGNIFICATION.
5. Start and finish the day’s work at the HIGHEST MAGNIFICATION
6. Always BEGIN examining a specimen with a LOW POWER objective.
7. Always BEGIN examining a specimen with a HIGHEST POWER objective
8. On the compound microscope, NEVER use the coarse adjustment knob with the high power or oil objective.
9. On the compound microscope, ALWAYS use the coarse adjustment knob with the high power or oil objective.
10. Unplug the microscope by pulling on the plug, not the cord.
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