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- Define the following words: replication fork, leading strand, lagging strand, Okazaki fragments, helicase, single strand, binding protein, primase, DNA polymerase 3, DNA polymerase 1, dna ligase and nucleaseList six types of DNA repair. Explain the basic features of each.What is the purpose and benefit of the polymerase chain reaction?
- How does DNA replication occur in a precise manner to ensure that identical genetic information is put into the new chromatid? See Figures 8.12 and 8.13. FIGURE 8.12 In DNA replication, the two polynucleotide strands uncoil, and each is a template for synthesizing a new strand. A replicated DNA molecule contains one new strand and one old strand. This mechanism is called semiconservative replication. FIGURE 8.13 A close-up look at the process of DNA replication. (a) As the strands uncoil, bases are added to the newly synthesized strand by complementary base pairing with bases in the template strand. The new bases are linked together by DNA polymerase. (b) DNA synthesis can proceed only in the 5 3 direction; newly synthesized DNA on one template strand is made in short segments and linked together by the enzyme DNA ligase.Why is DNA replication called semiconservative?If the sequence of one single strand of DNA is C-A-A-G-T-A-G-G-C-T, what is the sequence of the complementary strand? Describe the origin of each strand of the new double helices created after DNA replication. Why is DNA replication important to the growth and development of a multicellular organism? Place the following terms in the correct order from smallest to largest: Nucleosome, supercoils, coils, chromosome, DNA double helix
- What accounts for the amazingly accurate replication of DNA, which keeps the mutation rate low?Why is the replication of DNA referred to as a semiconservative process? What is the experimental evidence for the semiconservative nature of the process? What experimental results would you expect if replication of DNA were a conservative process?What are some of the ways that organisms use to ensure the fidelity of DNA replication? Why is it important that the fidelity of DNA replication is an evolutionary balance between faithful replication and the existence of some errors? Escherichia coli and other bacteria methylate adenines on the original strand to distinguish the original strand from the newly replicated strand of DNA. Why is this distinction important?
- Does E. coli chromosomal replication always start at one particular site? What is called? If you were given the DNA sequence of E. coli chromosome, would you be able to identify where E. coli chromosomal replication starts? What is the end of E. coli chromosome replication?If the template DNA sequence is 3' - CCC - ATA - GAG - AAA - 5' , then what is the corresponding daughter strand sequence? A. 3' - GGG - TAT - CTC - TTT - 5' B. 5' - GGG - TAT - CTC - TTT - 3' C. 3' - GGG - UAU - CUC - UUU - 5' D. 5' - GGG - UAU - CUC - UUU - 3'Which of the following types of DNA damage would be hardest to repair using the DNA repair pathways?A. Complete removal of three nucleotides in the middle of one strand.B. A covalent bond between a base on one strand and a base on the complementary strand.C. Incorporation of a sugar other than deoxyribose into one strand.D. Covalent attachment of a short polypeptide to a single base.E. A covalent bond between a base and a deoxyribose on the same strand. Please explain why it's B