Q: What is a mixed culture? A contaminated culture?
A: A microbiological culture, or microbial culture, is a technique for increasing microbial creatures…
Q: Since there are still bacteria left in pasteurized milk, why is it not routinely sterilized instead…
A: Hello! Since you have posted multiple questions, we are answering only first question. If you need…
Q: What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a…
A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…
Q: If an unknown bacteria was given that was gram-positive coccus, and the catalase test was performed,…
A: Gram-positive bacteria, such as Staphylococcus aureus, are bacteria that provide a positive response…
Q: Why do you have to boil the agar solution before dispensing it into tubes?
A: Agar is the medium which can used to grow the micro organisms in petri plates. The selected…
Q: In spread plate method, colonies form within the agar and agar surface. In pour plate method,…
A: Introduction Agar media is essential for the study of microorganisms and molecular biology, It is a…
Q: Why bleach disinfectant will the most effective against probiotic?
A: Probiotics are live microorganisms that have health benefits when consumed. These are usually…
Q: You will only isolate pure colony to inoculate again (sub-culturing) to newly prepared plate medium,…
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Why does a streaking method used to inoculate plates result in isolated colonies?
A: Streaking method is used to obtain pure colonies of microbes(bacteria) from a proper single species…
Q: Why is it important for a microbiology laboratory technician to use aseptic technique?
A: The majority of the microbes onto which scientists work in laboratories are known pathogens.
Q: What is the media used in Catalase test and its expected results?
A: Catalase is an enzyme that is found in the cell in most of the organism that protect the cell from…
Q: Which microbe requires serum components to be added to the growth medium?
A: Some microbes are nutritionally fastidious and hence needs some substances to be added to the growth…
Q: ou've been tasked with observing the motility of an UNKNOWN Bacteria isolated from a mixed culture.…
A: Bacteria is the tiny cellular living structure which belong to the Moneran kingdom. The bacteria…
Q: How will you prevent the occurrence of the bacteria present in the Rapid Strep A Test?
A: Answer: Rapid Strep A Test (Rapid strep test) is a test used to identify the presence of Strep A…
Q: procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar…
A: Aseptic Technique describe some of the ways that a laboratory can deal with the constant threat of…
Q: Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the…
A: The term sterilization refers to any process that eliminates, kills, removes, or deactivates all…
Q: If you had cultures of B. subtilis and E. aerogenes and forgot to label them, which of the following…
A: Some bacteria produce indole by deamination of amino acid tryptophan and shows positive indole test…
Q: Why is glucose not the choice of carbohydrate for most differential media used in the identification…
A: Bacilli present in the intestinal tract are rod shaped bacteria.for example, enteric bacteria…
Q: Can you please discuss the principles of using Motility Test Medium for the detection of bacterial…
A: Bacterial motility is usually demonstrated in a semi-solid agar medium. Motile bacteria ‘swarm' in…
Q: Where should a label be written on an agar plate?
A: The aseptic technique is applied in a laboratory setup to reduce the risk of contamination. It…
Q: Is the Peptone Iron Agar (PIA) a complex or defined medium? Explain based on its composition. Is the…
A: Depending on their composition, media can be classified as defined and undefined media. Defined…
Q: If SIM medium was used for motility determination for Proteus vulgaris, what noticeable change to…
A: What is motility? Motility is the ability of an organism to move by itself. Eucaryotic cells use…
Q: Explain why the media where the Staphylococcus aureus is plated has turned yellow but the media…
A: Mannitol Salt Agar (MSA) is a differential and selective medium. A member of the staphylococcus…
Q: Can you please help me describe the colony apperance on these two stains? Thank you! Growth on Blood…
A: The chocolate agar and the blood agar media both have the almost same composition. The only…
Q: What advantages do solid media offer for the isolation of microorganisms?
A: “Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: what is the culture media for gram-positive bacteria?
A: The culture media for gram positive bacteria is Mannitol salt agar . The presence of bile salts, as…
Q: Explain why it is important to use only a small amount of bacteria when preparing a smear
A:
Q: How do eosin-methylene blue (EMB) agar plates work? What organism(s) are they designed to detect?…
A: Several tests are performed in the laboratory in order to detect several bacterial agents as well as…
Q: How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial…
A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…
Q: Why does the oxidase test must be performed from blood agar or another medium without a fermentable…
A: Biochemical tests are used to identify microbial diversity based on variations in the biochemical…
Q: What is the result of Staphylococcus epidermidis in the TSI agar test?
A: Bacteria are microorganism that most commonly occur in the soil, air, water and in adverse…
Q: The difference in the colony appearance of bacteria cells growing on mannitol salt agar is detected…
A: Mannitol salt agar is a selective culture media that is used for the isolation of the pathogenic…
Q: What is the media used in Urease Test and expected results?
A: Urease test is defined as a biochemical test that distinguishes the basic alkaline fermentation of…
Q: What would be the expected outcome of an endospore stain test If the bacteria is a pathogenic…
A: Endospore stain is a differential stain that is used to image endospores in bacteria. A few genera…
Q: How can moist heat sterilization can sterilize antibiotic solution?
A: Moist heat sterilization is a technique that uses heated, increased pressure steam to sterilize an…
Q: How does the agar dilution method differ from streaking toobtain isolated colonies?
A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…
Q: Once the completed test establishes the presence of coliforms in the water sample, why might you…
A: Water is tasteless, transparent, inorganic. Colorless, and odorless chemical substance. Its chemical…
Q: how to find out the test is differential or selective in the microbiology lab test?
A: Many kinds of medias are used in microbial study. Two types of media having similar inferred names…
Q: Based on the growth you saw on your Lee’s Agar plate, what conclusions can you make about the…
A: Lee Agar is used to study the bacteria that can grow on yogurt. It is primarily used to count the…
Q: how could you determine if there is any contamination on your agar?
A: Agar consists of two polysaccharides, agarose and agaropectin, but agarose making up about 70% of…
Q: Explain why culture based biochemical and genetic testing would not be the ideal method to identify…
A: Microbial culture or microbiological culture is a technique in microbiology in which microorganisms…
Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…
A: Answer: Smear Preparation is the method in microbiology laboratories to place the small amount of…
Q: What microbe requires serum components to be added to the growth medium?
A: Introduction Microorganisms:- An organism that can be seen only through a microscope, They live in…
Q: selective agar media for gram-positive bacteria?
A: Phenyl Ethyl Alcohol Blood Agar is a selective media for gram positive bacteria.
Q: Other than the PYR test, what is another use of the PEP reagent?
A: PYR is a colorimetric method used to detect the presence of pyrolidonyl arylamidase activity in a…
Q: If testing had come back as oxidase negative, urease positive and growth on MacConkey agar, what…
A: MacConkey agar is a selective and differential culture medium used for the isolation and…
Q: Which culturing method provided the clearest results, the semisolid medium tube or the soft agar…
A: Agar is a jelly-like substance obtained from red algae. It is a mixture of two components namely…
Q: procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (2) agar…
A: A microorganism culture may be a check accustomed verify whether or not a bacterium or fungi are…
Q: Kligler’s iron agar and SIM are multiple test media.. Both media are stabbed but for different…
A: The liquid or solid substratum on which, the organ or cell explants can be grown. The medium…
Q: An unknown bacterium produces colorless colonies when inoculated onto an EMB plate. Predict what you…
A: EMB stands for Eosin Methylene Blue. It is a selective stain used for the growth of gram-negative…
Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the blood agar plates?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- When using Endo agar as the selective and differential media for the confirmed test, how would you know whether the test is positive for the presence of coli?Why go to the trouble of creating a master plate (why not simply plate the initial culture on both nutrient agar and glucose-salts agar)?Why does the oxidase test must be performed from blood agar or another medium without a fermentable sugar as MacConkey agar?
- What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?How does the agar dilution method differ from streaking toobtain isolated colonies?If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the pre-made plates by the time of the laboratory schedule?
- Why does a streaking method used to inoculate plates result in isolated colonies?In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?Why is dilution a necessary part of pure culture preparation?
- How will you prevent the occurrence of the bacteria present in the Rapid Strep A Test?What are the possible reasons why culture plates may have too many colonies despite performing serial dilutions and OD600 readings?You were given a mixed nutrient agar broth culture of bacteria 1a. How will you determine the different types of bacteria present in the mixed culture without using an agar plate 1b. How will you make a pure culture of these bacteria on a slant nutrient agar 1c. How will you identify these bacteria from the pure culture without using an agar plate