Why is CFU more applicable to a culture of streptococcus than to a culture of E.coli
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- Why is CFU more applicable to a culture of Streptococcus than to a culture of E. coli?Why can EMB agar be used to detect the presence of E. coli in particular? Explain the principle of detection of the organism using EMB agarWhy is it necessary to perform an enrichment procedure in isolating Staphylococcus aureus instead of directly plating it into a selective medium? What might happen if you omit potassium tellurite in using GCM?
- Why is it Koch's postulates cannot be used for the microbe E. Coli. What limitations does E.coli have?Why do cells need to be kept in the freezing devices first then transferred to liquid nitrogen storage in crypto preservation? In addition, why are antibiotics not used in the cell freezing media? Why FBS concentration higher than 10% (10% is the standard concentration in complete growth media) is recommended in cell freezing media?Why do we grow the bacteria in minimal media when testing for citrate utilization?
- What is the benefit of using LB (Lysogeny broth) medium for the growth of E. coli, Bacillus subtilis and Pseudomonas putida? Why ?Why is water tested for coliform bacteria rather than for pathogenic bacteria which may be present?Agar is a complex polysaccharide obtained from seaweed and cannot be digested by most microorganisms. Why does this property make agar an excellent solidifying agent for microbiological media?