Why is it important to use standards or to develop a standard curve in spectrophotometry?

Q: p between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.

A: A method to measure how much a chemical substance absorbs light by measuring the intensity of light…

Q: What is the diameter of field of view for the high-power objective in millimeters and micrometers?

A: A microscope is a device that allows the examining of small objects, including cells. The picture of…

Q: Why do you need to always focus slides initially using the x10 objective, even if you wish to use a…

A: A microscope is a device that magnifies an object so that it can be seen by the observer. A…

Q: The microscopic part that carries the objective lenses is the arm

A: A microscope is an instrument which allows us to see microscopic objects which are not visible to…

Q: What is reflectance spectrophotometry  What are it's general principles  Reflectance…

A: Answer 1 - Reflectance spectrophotometry is a spectrophotometric technique. Here, from the surface…

Q: What is the meaning of the different values given engraved on each objective?

A: Microscopes are used in viewing microscopic organisms, including bacteria, parasites, etc., and also…

Q: Differentiate between the concepts of magnifi cation, refraction, and resolution and explain how…

A: Magnification is the ability of the microorganism to make the tiny objects appear larger and more…

Q: Differentiate spectroscopy, spectrometry, and spectrophotometry

A: The study of the interaction of matter with electromagnetic radiation as a function of the…

Q: A radiograph is made using 40 mAs at 40-inch SID. If the image must be repeated at a 72-inch SID,…

A: Solved as below

Q: Which of the following is true about the relationship between depth of field and magnification?…

A: The depth of field refers to resolution in the longitudinal plane. It is measured as the distance…

Q: How do you identify which objective lens are you using to view your sample?

A: microscope objectives feature a range of magnifications from 1.25x to 150x. This is the first…

Q: If a circular object or specimen extends halfway across the scanning field, what isits diameter in…

A: Field of view is defined as the area of the slide that is seen when an observer looks through a…

Q: What is a Spectrogram

A: Spectrogram is a visual way of viewing the spectrum of frequencies of signal that varies with time.

Q: What is the relationship between the working distance of an objective lens and its magnification…

A: Microscope is an optical instrument that uses a lens or an arrangement of lenses to magnify an…

Q: ome spectrophotometers use wavelengths of light in the ultraviolet range. What are wavelengths of…

A: Spectrophotometer is an instrument that measures the amount of photons passing through them.

Q: If a microscope ocular lens has a power of 10x and the objective lens has a power of 20x then the…

A: Introduction: For imaging, optical microscopes combine ocular and objective lenses (eyepieces). The…

Q: If you observe a specimen under this 20X objective and the specimen spans one-quarter of the field…

A: A light microscope is common equipment in most laboratories. It comes with one or two eyepieces…

Q: What are the functions of the Fine Focus, Coarse Focus, Stage Clip, Rack Stop, Arm, Eyepiece,…

A: A microscope is an instrument used in laboratories to examine tiny objects that cannot be visualized…

Q: If the total magnification is 2000X with the use of a 10X ocular lens, what is the magnification of…

A: Answer- 200X

Q: Discuss the concept of spectrophotometry, colorimetery, and turbidimetry. Maximum of 150 words

A: Spectrophotometry is a part of electromagnetic spectroscopy worried about the quantitative…

Q: What is the rule for determining total magnification? A. Ocular lens magnification + objective lens…

A: Microscopy is a technique of incorporating microscopes to enlarge the size of objects (invisible to…

Q: The specimen that you are trying to find is very small and you cannot see it with scanning power.…

A: Microscopy is a field which involves using a microscope to view those specimens in enlarged size…

Q: How can you easily find specimens at higher magnifications after you found them at the lower…

A: Microscopes are commonly used in microbiology to provide a magnified image of the materials. Light…

Q: What is the total magnification possible with a 10x objective?

A: The microscope is used to view the objects that are not seen through the naked eye. Microscopes use…

Q: Differentiate between the concepts of magnification, refraction, andresolution and explain how they…

A: Microbes or microorganisms are small living organisms that can be observed with the aid of a…

Q: When using the 10x lens to view a specimen, what is the total magnification of the specimen to the…

A: When using the 10x lens to view a specimen, what is the total magnification of the specimen to the…

Q: Describe the conditions under which dark-field microscopy is the best choice for viewing specimens.

A: Darkfield microscopy is a method of microscopy in which the images are excluded from the unscattered…

Q: What is the significance of putting scale bar in photomicrographs?

A: Scale bar:A scale bar is an instrument or software that attaches to a slide or image when it is…

Q: Give two applications of Spectrophotometric analysis and briefly explain each

A: The spectrophotometer is an instrument that uses an electromagnetic spectrum. This instrument is…

Q: What are the functions of the Fine Focus, Coarse Focus, Stage Clip, Rack Stop, Arm, Eyepiece,…

A: A microscope is considered a laboratory instrument to observe microorganisms, small objects, cells,…

Q: If a darkfield condenser causes all light rays to bypass the objective, where does the light come…

A: In bright-field microscopy, thin and transparent objects cannot be seen properly because of the…

Q: when you want to compute for the magnification of the specimen, what will you do with the eyepiece…

A: Introduction   There are various types of microscopes available in the market which have different…

Q: Discuss if visual observations match with spectrophotometric analysis results.

A:

Q: Explain the difference between a transmission electronmicrograph (TEM) and a scanning electron…

A: A microscope is a laboratory instrument that is used to examine objects that are too small for the…

Q: Can someone explain the concept behind a sample that lies in the UV range in a spectrophotometer?…

A: UV spectroscopy or UV–visible spectrophotometry refers to absorption spectroscopy or reflectance…

Q: Introduce and explain comprehensively  the process of calculating the final magnification of…

A: A microscope is a device that magnifies an object so that the spectator can see it. A microscope is…

Q: Do the calibration factor computed varies according to different magnification (objective lenses…

A: By simply accounting for the inaccuracy, the Correction Factor allows us to attain more accurate…

Q: Total magnification of a specimen using the high objective and the ocular lens is

A: B) 40Ox 400 x is the typical magnification of an ocular lens.

Q: What is the principle behind a UV-Vis spectrophotometer, and what are its key components, and how…

A: UV-vis spectrophotometry or UV spectroscopy types of absorption spectroscopy or reflectance…

Q: The numerical aperture of the an objective lens:   indicates magnification   indicates the…

A: Microscopy techniques enable users to observe specimens with much clarity and resolution. Various…

Q: Explain the difference between resolution and mag­nification.

A: Introduction :- The term'resolution' is used in microscopy to describe a microscope's ability to…

Q: What are the significance of UV/Vis spectrophotometer in the experiment complexation.

A: UV/VIS spectrophotometer is the technique used to determine the optical properties  such as…

Q: In a tabular form, enumerate the different components of a spectrophotometer and explain its…

A: A spectrophotometer is an analytical equipment that is used to measure the absorbance of light by…

Q: Define magnification and resolution

A: In biology, i can define the term magnification and resolution on the basis of microscopy.

Q: What is the purpose of setting the spectrophotometer to zero absorbance using a blank solution?

A: Spectroscopy is a procedure utilized for the evaluation, portrayal, and basic investigation of a…

Q: Why must the entire calibration procedure be performed for each objective?

A: An ocular micrometer has a circular "glass disk" that fits in a microscope eyepiece and possesses a…

Q: Provide a list of the names of the devices and technologies used in Dynamic Light Scattering applied…

A: Dynamic Light Scattering (DLS) employs a measurement method to identify the particle size in…

Q: what is experimental error that may occur when reading spectrophotmeter

A: Spectrophotometry is a technique for determining how much light a chemical substance receives by…

Q: Do the calibration factor computed varies according to different magnification (objective lenses…

A: A compound light microscope is a type of microscope which has own light source and more than one…

Scientific Notation

We have to deal with quantities that are extremely large or very small. For example- I may have 6,500,000,000 red blood cells, or the diameter of an iron atom is 0.000000011 inches. Numbers with many zeros can be inexpedient to work with, so scientists use scientific notation. It represents very large numbers or very small numbers in a simple form. We know that whole numbers can be extended to infinity, but we cannot write such large numbers on paper. Numbers that present in millions of places after the decimal point need to be represented simply. Thus, it is difficult to represent a few numbers in their extended form. Therefore, we use scientific notation.