Why is it necessary to use oil in conjunction with the oil immersion lens? Check All That Apply The oil reduces the scattering of light The oil minimizes the numerical aperture The oil improves the resolving power of the microscope
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- 1. after going through all 4 objectives, which would give you the largest field of view? greatest depth of focus? why? 2. how do you calculate the total magnification of a specimen? show sampleA&P Lab; what are the steps to take before selecting a slide for viewing, using the V- Scope?3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. A.The cell is being viewed under high power. Scanning power objective=4x; Low power objective= 10x; High power objective= 40x; Eyepiece= 5x; Low power field of view (FOV)= 4.2 mm
- The FOV on low power of a microscope is measured to be 4.5mm. Using the chart below and the above listed formulas calculate: FOvx = FOvlow X Maglow Magx S.S. = FOvx # times the specimen fits across the FOv Total Mag = Ocular lens x objective lens a) What is the FOV on High Power? (Magnification on High power is 40x) Can you please include the units too. asap please.How will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensityUsing the scanning (4x) objective and the metric ruler, record the number of millimeters you see along with the letter “e.” Your value: 2 millimeters Convert the figure you attained to micrometers (1 millimeter = 1,000 micrometers). This is the diameter of the field of view for the low-power objective (LPD). The field of view is the circular field you see when you look through the oculars. The field of view changes at different magnifications. Your value (LPD): 2,000 micrometers -please help me with the problem in the picture.
- How does the numerical aperture of an objective lens affect resolution? Question options: Numerical aperture is the optical plane visible for each objective lens Numerical aperture decreases the amount of excess light on a specimen increasing the resolution and clarity Numerical aperture is the loss of light due to changes in density between the specimen and the objective lens Numerical aperture is a measure of the amount of light an objective lens can accept producing higher resolution and allowing smaller structures to be visualized with greater clarityAnswer all the questions. 1. How do you calculate total magnification? 2. Which lens requires the use of cedarwood oil? Why is it important to use oil when focusing under this lens?Here are the materials and method for the basic spectrophotometer experiment Materials:1. Paper template2. Scissor3. Light source (i.e. torchlight, portable light)4. Paperboard (black colour)5. Unused compact disc (CD)6. Camera detector (i.e. webcam, laptop, mobile phone) Methods: 1. Make a foldable paper spectrophotometer using the paper template and followthe instruction as in https://publiclab.org/sites/default/files/8.5x11minispec3.8.pdf2. Then, mounted the paper spectrophotometer to the camera detector.3. Download any freely available spectral analyzer.4. Test and observe the wavelength with and without the presence of a light source.5. Finally, observe the wavelength with a different transparent colour sheets (you canchoose any colour). Troubleshooting/ problem(s) encountered for basic spectrophotometer experiment.
- Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's Law3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mmA student using a microscope measured the low power field diameter to be 7.1mm. The student then switched from the low power (4x) objective lens to the medium-power (10x) objective lens. Assuming a 10 x ocular (eyepiece) lens, the medium power field diameter is ____ um um is micrometers