Why it is possible/ applicable to use recrystallization and vacuum filtration with Luminol? Cite some reasons.(Explain in at least 1 paragraph)
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- Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayPlease explain why is 70% ethanol used as an effective disinfecting solution in labs? Why not 95% ethanol? Please include references.In preparing bacterial smear, why do we need to pass the slide over the flame? Enumerate the advantages derived from staining bacteria. In Gram staining method, list the reagents and state their role for each step of the procedure? Conclusion?
- Your instructor asks you to isolate and identify the organisms in an unknown culture. You find that the culture contains two gramnegative bacilli that produce swarming colonies. What biochemical test would you use to identify the bacilli? Justify your answer.Why is it best to use sterile distilled water in the preparation of microbial suspension and dry smears for slide preparation? What possible error could happen if a glass slide is reused for slide preparation?The acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in performing the acid-fast stain technique because of its thin cell wall. However, if this organism is used for Gram-stain, it can also take the Gram stain reaction. Why is this so?
- Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?Why should the use of nichrome or iron containing loop/wire be avoided as an inoculating device when performing the oxidase test in microbiology lab?For preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise when the slide is heated in a flame?
- ou make a smear of E. coli and then simple stain the slide with methylene blue. If you failed to use correct aseptic technique by not sterilizing the loop before picking up the bacterial sample for the smear, what results may be seen under the microscope?Agar is a solidifying agent used in media preparation. How and why does the agar concentration in semisolid media differ from conventional solid media?In Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?