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Why should your solvent be evaporated from the sample placed on the sterile disk before you place it your petri dish?
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- You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is the dilution factor. Show workYou have 2 mL of bacterial culture. How would you make a 10^-2 dilution using the entire culture volumeIf you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar plate, what is the final dilution?
- You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is the dilution factor?What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a sterile petri dish and then added molten agar to make a pour plate?To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a final volume of 5 ml. How did you calculate it?. Single line text.
- Why is dilution a necessary part of pure culture preparation?During the preparation of the capsule stain, the slide used to spread the India ink/bacteria mixture is discarded in the disinfectant solution. Why?Dilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you add to the new media?
- In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?What is the physical appearance (solid, liquid, semi-solid) of following media: agar plateIf you diluted 200 uL of your culture into 1800 uL of water what is your dilution factor? (Show your work)
