Why would gel filtration chromatography be the best method for purifying an antigen for injection into a mouse to generate monoclonal antibodies? Antigens can easily be purified by charge and size when coated with SDS O Antigens will separate by size going through the column beads and their holes Antigens can be centrifuged through a column and pellet at specific speeds O Organelles containing the antigen will have a specific buoyancy and separate in a gradient Antibodies can be used to detect the antigen on a western blot

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A:

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Violence

Violence is defined as a physical act that causes or is intended to cause harm. Violence can cause physical, emotional, or both types of harm. Violence is defined by the World Health Organization as the intended use of physical force and power that causes threat or harm against oneself, another person, or against a community or group, and has the potential to cause damage, death, mental distress, maldevelopment, or poverty.