wo components in an HPLC separation have retention times that differ by 22 s. The first peak elutes in 10.5 min and the peak widths are approximately equal. Use a spreadsheet to find the minimum number of theoretical plates needed to achieve the following resolution, Rs, values: 0.50, 0.75, 0.90, 1.0, 1.10, 1.25, 1.50, 1.75, 2.0, and 2.5. How would the results change if peak 2 were twice as broad as peak 1?
wo components in an HPLC separation have retention times that differ by 22 s. The first peak elutes in 10.5 min and the peak widths are approximately equal. Use a spreadsheet to find the minimum number of theoretical plates needed to achieve the following resolution, Rs, values: 0.50, 0.75, 0.90, 1.0, 1.10, 1.25, 1.50, 1.75, 2.0, and 2.5. How would the results change if peak 2 were twice as broad as peak 1?
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter27: Gas Chromatography
Section: Chapter Questions
Problem 27.23QAP
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Question
Two components in an HPLC separation have retention times that
differ by 22 s. The first peak elutes in 10.5 min and the peak
widths are approximately equal. Use a spreadsheet to find the
minimum number of theoretical plates needed to achieve the
following resolution, Rs, values: 0.50, 0.75, 0.90, 1.0, 1.10, 1.25,
1.50, 1.75, 2.0, and 2.5. How would the results change if peak 2
were twice as broad as peak 1?
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