You are added 60mL of soil slurry to 540mL of sterile water. You can transferred 1000uL of that dilution to 9 mL of water. You then prepared a 10-7 dilution from that tube. You finally transfer 1 uL of that to a TSA plate. After 48 hours, you counted 32 colonies on quarter of the plate. (A) How many cells/mL were present in the original soil sample? (B) How many colonies would be present on the plate if you would have plated 1mL of the last sample? (C) How many milliliters would you need to prepare a 10-4 from 100uL of sample?

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)
6th Edition
ISBN:9781305964792
Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa
Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa
Chapter30: Assisting With Minor Surgery
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You are added 60mL of soil slurry to 540mL of sterile water. You can transferred 1000uL of
that dilution to 9 mL of water. You then prepared a 10-7 dilution from that tube. You finally
transfer 1 uL of that to a TSA plate. After 48 hours, you counted 32 colonies on quarter of
the plate.
(A) How many cells/mL were present in the original soil sample?
(B) How many colonies would be present on the plate if you would have plated 1mL of the
last sample?
(C) How many milliliters would you need to prepare a 10-4 from 100uL of sample?
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