You are centrifuging a test tube of spherical cells in a buffer solution. The buffer viscosity and density are 0.02 Pa-s and 1.0 g/cm³; the cells have a diameter 4.5 µm and density is 1.2 g/cm3. The centrifuge rotates at 4000 rpm, with sample holders placed 10 cm from the rotation axis. Which of the following changes will result in having your samples sediment in the least amount of time? O Increasing the viscosity of the buffer to 0.04 Pa s. Doubling the length of the test tube. O Reducing the buffer density to 0.8 g/cm³. Increasing the rotation rate to 8000 rpm. Increasing the distance of the sample holder to 20 cm.
You are centrifuging a test tube of spherical cells in a buffer solution. The buffer viscosity and density are 0.02 Pa-s and 1.0 g/cm³; the cells have a diameter 4.5 µm and density is 1.2 g/cm3. The centrifuge rotates at 4000 rpm, with sample holders placed 10 cm from the rotation axis. Which of the following changes will result in having your samples sediment in the least amount of time? O Increasing the viscosity of the buffer to 0.04 Pa s. Doubling the length of the test tube. O Reducing the buffer density to 0.8 g/cm³. Increasing the rotation rate to 8000 rpm. Increasing the distance of the sample holder to 20 cm.
Chapter8: Sampling, Standardization, And Calibration
Section: Chapter Questions
Problem 8.7QAP
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