You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is the dilution factor

Why should your solvent be evaporated from the sample placed on the sterile disk before you place it your petri dish?

You place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix.  You take 1 ml from bottle A and transfer it to bottle B, which contains 99 ml of sterile water.  What is the dilution of the bacterial culture in bottle B?

Your first task in the lab is to learn how to culture these cells and begin expanding the culture (increasing the # of cells) so that you may later conduct experiments on them.  You take two aliquots, each with an equal number of cells, and place them in two different culture vessels, one is a sealed airtight flask (culture 1) and the other is an open topped culture dish (culture 2). You provide both with the same growth media that contains their preferred fuel source, glucose.      Are the cells in cultures 1 and 2 deriving all their energy in the same way?  (Yes or No) Explain why or why not.  Be sure to name the specific processes by which each culture is deriving its energy.

You have found that the D-value (decimal reduction value) of an antimicrobial agent to be 4 minutes when the agent was exposed to a bacterial culture having an initial total viable cel1 count of 10° CFU/mL. After 4 minutes upon addition of the antimicrobial agent, what would have been the total viable count of the culture in this experiment? A) O 10° CFU/mL B) O 10$ CFU/mL C) O 10° CFU/mL D) O 10' CFU/mL