You conduct the following serial dilution:  Step 1: In tube 1, you add 100 ul of plasma to 900 ul of distilled water. Step 2: In tube 2, you then take 100 ul from tube 1 and add 900 ul of distilled water.Step 3: In tube 3, you then take 100 ul from tube 2 and add 900 ul of distilled water. What is the final/total dilution factor in tube 3 at the end of step 3?

Question
Asked Sep 30, 2019
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You conduct the following serial dilution:  

Step 1: In tube 1, you add 100 ul of plasma to 900 ul of distilled water. 

Step 2: In tube 2, you then take 100 ul from tube 1 and add 900 ul of distilled water.

Step 3: In tube 3, you then take 100 ul from tube 2 and add 900 ul of distilled water.

 

What is the final/total dilution factor in tube 3 at the end of step 3?

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Expert Answer

Step 1

serial dilution is any dilution in which the concentration decreases by the same dilutions in each successive step.

In serial dilutions, you multiply the dilution factors for each step.

The dilution factor or the dilution is the initial volume divided by the final volume.

DF=Vi/Vf

Where, DF – dilution factor, Vi  - initial volume, and Vf – final volume.

Step 2

1st step: the initial volume is 100 µl of plasma which is mixed with 900 µl of distilled water. Therefore, the Vi is 100 µl and the Vf is 1000 µl.

Dilution factor = initial volume / final volume

                              100 / 1000

                  ...

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