You dilute 1 mL of bacterial culture with 99 mL of water.  What is the resulting dilution factor?

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Q: You prepared a 7x 10^5x dilution from your bacterial culture, plated 0.2 ml of it on a Petridish and…

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Q: You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is…

A: The following are needed for calculating the dilution factor  1) Original volume of  solution we…

Q: If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar…

A: Making a solution thinner or less dense is the procedure of dilution. Serial dilutions (also known…

Q: how many bacteria/ml of the diluted broth would be present on average

A: 37 bacteria/ml of the diluted broth would be present on average.

Q: Describe one error in the staining procedure that would result in a Gram-positive bacterium…

A: Gram staining is a differential staining technique that differentiates between a gram-positive and a…

Q: You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell…

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Q: During the preparation of the capsule stain, the slide used to spread the India ink/bacteria mixture…

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Q: If you altered the conditions under which bacterial growth normally occurs (i.e. increase the…

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Q: Why do we use the term CFU (colony forming unit) instead of reporting bacteria per ml? Explain.

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Q: describe each dosage form and enumerate and describe each kind. EMULSIONS-3 KINDS SOLUTIONS-3 KINDS

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Q: Explain how to perform a serial dilution

A: Serial dilution is stepwise dilution of a solution keeping the dilution factor constant in each…

Q: Heat-labile material to be introduced to culture medium was filtered using membrane filters with…

A: given that the heat labile material which is introduced to culture media is filtered using a…

Q: If there is 50 bacterial colonies on a 1:1000 dilution streak plate. How many cfu/mL are there?

A: Introduction: A CFU stands for colony-forming units. It is a unit that we use for estimating the…

Q: What is the purpose of adding sodium bicarbonate to a culture medium?

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Q: why In spread-plate technique, the bacterial suspension volume  should not exceed 0.1ml.

A: Spread plate method is a technique which uses a Petri plate or dish in which a growth medium or gar…

Q: Measurement of Bacterial Growth in LB, pH 3.2 Plot of Absorbance vs. Time (mins) 4"c 37°C --45°C 1.2…

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Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

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Q: Measurement of Bacterial Growth in LB, pH 3.2 Plot of Absorbance vs. Time (mins) c 37c 45'c 0.6 0.4…

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Q: To measure the culture temperature, why is the thermometer placed in a tube separate from the…

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Q: If I add 10 microliters of a culture at 106 cells/mL to 990 microliters of nutrient broth, what is…

A: Cell density refers to number of cells present per unit volume. It is usually expressed in cells/mL.

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Q: Why is it necessary to store the molten agar in a water bath at 50 degrees Celsius?

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Q: How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume?…

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Q: Assuming you had a bacterial culture with a concentratoion of 2x10^9 bacteria/mL, whaat volume would…

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Q: ou make a smear of E. coli and then simple stain the slide with methylene blue. If you failed to use…

A: The Gram stain is a differential staining method used for classifying bacteria into either…

Q: If you started with a bacterial concentration of 2,000,000 bacteria/ml and wanted to plate out 40…

A: In the question it is asked that they want to plate out 40 bacteria, and this count comes under the…

Q: How can you account different diffusion rates of three dyes through the agarose media in the Petri…

A: Agar is used as a support medium for bacterial growth and culture in labs.This is extracted from sea…

Q: You have a starter culture containing 8 x 109 cells per mL, from which you take 10 mL to inoculate a…

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Q: Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram…

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Q: You are asked to add 2 mL of bacterial culture to 98 mL of water. What is your dilution factor? Show…

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Q: You are asked to make a 1:1000 dilution of a bacterial culture in a final volume of 5000 mL. What…

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Q: Describe how you would make 10 ml of a 1:500 dilution of bacteria by performing TWO SEPARATE…

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Q: In preparing bacterial smear, why do we need to pass the slide over the flame?

A: since this is a multi-part question, so I am answering only 1st part. please upload other questions…

Q: To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a…

A: Given Values: Volume of the 16 hour culture = 500 μl Final Volume of the culture media = 5 ml

Q: How would you inoculate a plate to get a 1:10 dilution? A 1:100 dilution?

A: Inoculation is defined as the process of introduction of microorganisms into a culture for their…

Q: Dilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you…

A: Culture consists of appropriate substances which provides the growth to the desired organisms.

Bacterial Morphology

The bacteria are prokaryotic organisms that are single-celled, and are found to exist as free-living and possess a microscopic size. The morphology is found to vary in the bacteria, where some of them are identified as individual organisms and the others are detected as colonies. The size and shape of the bacterial cell also represent its morphology. 

Bacterial cell structure

Bacteria are single-celled, tiny creatures that may enter healthy tissues and grow rapidly. Bacteria are microscopic organisms that are tiny and unicellular. These are members of the prokaryote kingdom. They live in water, air, soil, and all-natural environments. They are used in industrial and therapeutic processes, and they support a wide range of plant and animal life. The first organism to appear on the planet. Bacteria-like creatures are the oldest known fossils. Bacteria can consume a wide range of organic and inorganic elements, and some may even survive in harsh conditions.