You have been provided with stock solutions of: stock A:     0.06 M sodium pyrophosphate buffer pH 8.5 stock B:     3 M ethanol stock C:     0.015 M NAD+ stock D:     milli Q water Determine the volume you will need of each solution to prepare a buffer of with a final volume of 60 mL containing 10 mM sodium pyrophosphate pH 8.5, 100 mM ethanol, 1 mM NAD+. i.e. volume of stock A = _________mL volume of stock B = _________mL volume of stock C = _________mL volume of stock D = _________mL

Aquaculture Science
3rd Edition
ISBN:9781133558347
Author:Parker
Publisher:Parker
Chapter11: Health Of Aquatic Animals
Section: Chapter Questions
Problem 3KA
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You have been provided with stock solutions of:

stock A:     0.06 M sodium pyrophosphate buffer pH 8.5

stock B:     3 M ethanol

stock C:     0.015 M NAD+

stock D:     milli Q water

Determine the volume you will need of each solution to prepare a buffer of with a final volume of 60 mL containing 10 mM sodium pyrophosphate pH 8.5, 100 mM ethanol, 1 mM NAD+. i.e.

volume of stock A = _________mL

volume of stock B = _________mL

volume of stock C = _________mL

volume of stock D = _________mL

Show your calculations to arrive at your answers.

 

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