Question
Asked Sep 13, 2019
50 views

You need to prepare medium for your culture cells. Your salt solution is supplied at a 10X concentration but needs to be 2X for use. You also need to add fetal bovine serum for a final concentration of 8%. What would you add of each for the correct final concentrations in 5 L of media?

check_circle

Expert Answer

Step 1

The question asks to find the final concentrations of the following in 5 L of media:

  1. The salt solution
  2. The fetal bovine serum
Step 2

Given data is placed on the board:

help_outline

Image Transcriptionclose

For 5liters: Given concentration of Salt 10x FBS 100% Final concentration of Salt 2x FBS 8%

fullscreen
Step 3

Fetal bovine serum or FBS is defined as a by-product of the dairy production. It is made from dairy cattle blood that is collected at the slaughterhouses that delivers meat for human consumption. FBS is usually a majority of a...

help_outline

Image Transcriptionclose

For 1 Litre: Concentration from 10x to 2x =200 mL salt +800mL culture =1000 mL For 5 Litres: Concentration from 10x to 2x = 5x(200 mL salt +800mL culture) =1000 mL salt +4000 mL culture = 5000 mL

fullscreen

Want to see the full answer?

See Solution

Check out a sample Q&A here.

Want to see this answer and more?

Solutions are written by subject experts who are available 24/7. Questions are typically answered within 1 hour.*

See Solution
*Response times may vary by subject and question.

Related Biology Q&A

Find answers to questions asked by student like you
Show more Q&A
add
question_answer

Q: what two hormones of the thyroid and parathyroid glands are antagonistic?

A: The thyroid gland is a butterfly shaped gland which is located in front of the trachea and just belo...

question_answer

Q: How many months would it take two water hyacinths to cover a pond that is 32m? Assume a three month ...

A: The doubling time of an organism is defined as the amount of time taken by the population to double ...

question_answer

Q: what was the first element formed in the universe

A: The Big Bang Theory is the leading explanation about how the universe began. It explains that the un...

question_answer

Q: First 3 only

A: (A) Gene flow: It is known as the transfer of genetic variation or the exchange of alleles between p...

question_answer

Q: In an experiment you do a chi-square test comparing observed and expected progeny. Your calculated c...

A: A chi-squared test is a statistical hypothesis test that measures how expectations compare to the ac...

question_answer

Q: What factors affect the distribution of ventilatioin and perfusion of the lungs?

A: Introduction:Respiration is the process of gaseous exchange taking place in the lungs. Each cell of ...

question_answer

Q: A filamentous organism has been isolated from decomposing organic matter. This organism has a cell w...

A: It is given that a filamentous organism has been isolated from decomposing organic mattter. Filament...

question_answer

Q: How will treatment for the second tuberculosis infection likely differ from the first round?

A: The Mycobacterium tuberculosis is the causative agent to cause tuberculosis infection. The tuberculo...

question_answer

Q: Simple squamous

A: Epithelial tissueEpithelial tissue is tissue which covers the body of the surface of lines of the bo...