) You perform column chromatography on a sample of clove oil and collect a total of 16 fractions. You analyze each fraction by TLC using four TLC plates. One plate has fractions 1-4, one has fractions 5-8, one has fractions 9-12, and one has fractions 13-16. After developing all of your TLC plates, however, you realize that you forgot to label the TLC plates with the fraction numbers. Label the TLC plates below with the fraction that they contain (1-4, 5-8, 9-12, 13-16). •.. --------- -++++- ----F-F-F-

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
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Chapter33: High-performance Liquid Chromatography
Section: Chapter Questions
Problem 33.1QAP
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please answer f 

3. The main components of clove oil are eugenol, eugenol acetate, and b-caryophyllene.
но
eugenol
EMgeRAlacetate
B-caryophyllene
a) Rank the compounds from least polar to most polar
b) After analyzing clove oil by TLC using a 10% acetone in hexanes mobile phase, you obtain the following
TLC results. Identify the compound that corresponds with each spot.
5 cm
2.8 cm
c) Calculate the Ri value of the middle spot. Show your work
d) What would happen to the R of the spots if you increased the polarity of the mobile phase? Explain
e) When separating the compounds by column chromatography, which compound would you expect
first? Which compound would you expect to elute last?
elute
Draft
f) You perform column chromatography on a sample of clove oil and collect a total of 16 fractions. You
analyze each fraction by TLC using four TLC plates. One plate has fractions 1-4, one has fractions 5-8,
one has fractions 9-12, and one has fractions 13-16. After developing all of your TLC plates, however, you
realize that you forgot to label the TLC plates with the fraction numbers. Label the TLC plates below with
the fraction that they contain (1-4, 5-8, 9-12, 13-16).
•...
•..
----------
--++-F-
----F-F-
---------
Transcribed Image Text:3. The main components of clove oil are eugenol, eugenol acetate, and b-caryophyllene. но eugenol EMgeRAlacetate B-caryophyllene a) Rank the compounds from least polar to most polar b) After analyzing clove oil by TLC using a 10% acetone in hexanes mobile phase, you obtain the following TLC results. Identify the compound that corresponds with each spot. 5 cm 2.8 cm c) Calculate the Ri value of the middle spot. Show your work d) What would happen to the R of the spots if you increased the polarity of the mobile phase? Explain e) When separating the compounds by column chromatography, which compound would you expect first? Which compound would you expect to elute last? elute Draft f) You perform column chromatography on a sample of clove oil and collect a total of 16 fractions. You analyze each fraction by TLC using four TLC plates. One plate has fractions 1-4, one has fractions 5-8, one has fractions 9-12, and one has fractions 13-16. After developing all of your TLC plates, however, you realize that you forgot to label the TLC plates with the fraction numbers. Label the TLC plates below with the fraction that they contain (1-4, 5-8, 9-12, 13-16). •... •.. ---------- --++-F- ----F-F- ---------
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