Question

You want to identify and compare the gene expression profile of several genes in a disease. Will you be able achieve this using Q-PCR technique? 

Can you identify a potential mutation (the nucleotide change) within a gene sequence using conventional PCR? If not, then what approach you should take? 

Expert Answer

Want to see the step-by-step answer?

Check out a sample Q&A here.

Want to see this answer and more?

Experts are waiting 24/7 to provide step-by-step solutions in as fast as 30 minutes!*

*Response times may vary by subject and question complexity. Median response time is 34 minutes for paid subscribers and may be longer for promotional offers.
Tagged in
Science
Biochemistry

Gene expressions

Related Biochemistry Q&A

Find answers to questions asked by students like you.

Q: How does horseradish peroxidase (HRP) contribute to creating the final colored sample? O A. HRP is a...

A: In biochemistry techniques, the enzyme horseradish peroxidase (HRP), which is isolated from the root...

Q: The diploid chromosome number for the somatic cells of a frog is 26. What would that number be after...

A: Meiosis is important because it ensures that all organisms produced via sexual reproduction contain ...

Q: Starch synthase in plants contains a single active site that accommodatesADP–glucose. However, cellu...

A:  Starch synthase is an enzyme catalyzing the reaction; ADP-glucose + (1,4-alpha-D-glucosyl)n {\disp...

Q: What is the significance of abnormality of high concentration of uric acid in the urine?

A: A uric acid test measures the amount of uric acid in the body. Uric acid is a chemical that is produ...

Q: How does the oxidation of a saturated fatty acid differ from the oxidation of an unsaturated fatty a...

A: Saturated fatty acids are the basic type of fats with linear chains of CH2 groups connected with a t...

Q: Discuss the metabolism on how ketone bodies, glucose, pentose, albumin, bile and occult blood is for...

A: Metabolism is the complete quantity of biochemical reactions involved in preserving the cells of an ...

Q: Digestion of plant materials generates phytanate (derived from chlorophyllmolecules; (a) Explain why...

A: Phytanate or Phytanic acid is a branched chain fatty acid that humans can obtain through consumption...

Q: Cyanobacteria contain carboxysomes, which consist of a protein shell that encloses RuBP carboxylase ...

A: The proteinaceous organelles that play an important role in improving the carbon fixation in proteob...

Q: Identify the type of structural conformation found within the botulinum toxin molecule.

A: Clostridium botulinum produces the botulinum neurotoxin.

Q: An unknown substance containing only C, H, and O was isolated from goose liver. A 0.423 g sample pro...

A: Given, Mass of CO2 = 0.620 g Mass of H2O = 0.254 g Total mass of sample = 0.423 g Molar mass of CO2 ...

Q: Describe the production of dNTPs from their corresponding NDPs.

A: Nucleosides are a combination of a nitrogen base with pentose sugar. The nitrogen base combines with...

Q: Ison, asse oxygen; (4) ATP synthase; and finally, do you predict it to be fatal to humans?. 1. Cyani...

A: Cyanide poisoning occurs from exposure to any forms of Cyanide. A range of serious symptoms like diz...

Q: L(24 points) Explain How is the Michaelis constant defined, and what does a low or high value for Km...

A: Enzymes acts as the biological catalysts. They regulates the rate at which chemical reactions procee...

Q: a. What is a genetic mutation? How do genetic mutations differ fromsomatic mutations?  b. What are m...

A: “Since you have posted a question with multiple sub-parts, we will solve first three subparts for yo...

Q: In some forms of diabetes, a mutation in the b subunit of the insulin receptor abolishes the enzymat...

A: Insulin is a peptide hormone developed as the body's primary anabolic hormone by pancreatic beta cel...

Q: Select all the possible non-reducing sugar/s from the list below. CHO CH2OH но но но -OH OH он но но...

A: Carbohydrates  are the polyhydroxy ketones or polyhydroxy aldehydes or their derivatives. In additi...

Q: A eukaryotic cell carrying out transcription and RNA processing is incubated with 32P-labeled ATP. W...

A: Introduction: The process in which RNA is synthesized from DNA is known as transcription. Therefore,...

Q: For the mitochondrial membrane described in pic, how many protons must be transported to provide eno...

A: Reduced coenzymes NADH and FADH2 retains most of the free energy produced during the process glucose...

Q: How can you recognize an enzyme that does not display Michaelis–Menten kinetics?

A: Enzymes are proteins that catalysis a biochemical reaction. Enzymes are restored after the reaction....

Q: Genetically engineered mRNAs that code for a stretch of basic residues, such as poly(Lys), induce tr...

A: Genetics is the branch of biology, which deals with the study of genes, their pattern of inheritance...

Q: An example of competitive inhibition of an enzyme is step ____ of glycolysis; an example of noncompe...

A: Glycolysis is defined as how one glucose molecule will be converted into two pyruvate molecules, two...

Q: To store glucose as the polysaccharide ___________________, __________________ is first converted to...

A: Introduction: Polysaccharides consist of repeat units of monosaccharides or their derivatives which ...

Q: How many moles of ATP could theoretically be synthesized under standard conditions using the energy ...

A: Photon is an elementary particle. These particles are massless and in vacuum, they move at the speed...

Q: What is the signifi cance of the different DNA-binding properties of prokaryotic RNA polymerase core...

A: RNA polymerase (RNAP) is the enzyme that transcribes the genetic information in DNA to RNA. Structur...

Q: If G6P is labeled at its C2 position, where will the label appear in the products of the pentose pho...

A: The alternative pathway of glucose oxidation is the pentose phosphate pathway that includes two phas...

Q: a) State the functions of the subunits of RNA polymerase and describe transcription initiation. b)Dr...

A: Operon system present in bacteria i.e in prokaryotes only.

Q: Describe the regulation of the lac operon by lac repressor and CAP.

A: Operon acts as a single regulated unit having one or more structural genes, a promoter gene, a regul...

Q: True/False Questions Snake venom contains phospholipases which cause hemolysis of the red blood cel...

A: Hi! Thank you for the questions. As you have posted multiple questions, I will be answering the firs...

Q: Convert each of the following chair conformations to an open-chain from and to a Fischer projection....

A: it is the structure of Glucose

Q: Part a) and b) have already resolved and part c and d tobe resolved.  a. What is a genetic mutation?...

A: DNA transposons are powerful forces of genetic change. They move from one genomic location to anothe...

Q: How did inhibitors reveal the order of electron transport?

A: Oxidative phosphorylation is the step in cellular respiration in which reduced coenzymes NADH and FA...

Q: Consider the transport of K* ions from a surrounding fluid (where [K'] = 30 mM) into a cell (where [...

A: A spontaneous reaction or a favorable reaction is characterized by negative value of Standard Gibbs ...

Q: A biochemist carefully measures the molarity of salt in 56. mL of photobacterium cell growth medium ...

A: GIVEN: The initial volume of the photobacterium cell growth medium (V1)= 56 mL Concentration of the ...