You wish to produce a high-value protein using recombinant DNA technology. Would you try to develop a chemical defined medium or a complex medium? Why? Explain what semiconservative replication means. Give characteristic dimensions for each of these organisms: E. coli Yeast (S. cerevisiae) Liver cell (hepatocyte) Plant cell
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
You wish to produce a high-value protein using recombinant DNA technology. Would you try to develop a chemical defined medium or a complex medium? Why? Explain what semiconservative replication means. Give characteristic dimensions for each of these organisms: E. coli Yeast (S. cerevisiae) Liver cell (hepatocyte) Plant cell .What are the differences in cell envelope structure between gram-negative and gram-positive bacteria? These differences become important if you wish to genetically engineer bacteria to excrete proteins into the extracellular fluid.
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