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Your lab-mate observes a large amount of streaking and blurring of their samples when they visualize the TLC plate under UV light. What are two possible solutions to this problem?

EBK A SMALL SCALE APPROACH TO ORGANIC L

4th Edition

ISBN:9781305446021

Author:Lampman

Publisher:Lampman

Chapter10: Tlc Analysis Of Analgesic Drugs

Section: Chapter Questions

Problem 2Q

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Your lab-mate observes a large amount of streaking and blurring of their samples when
they visualize the TLC plate under UV light. What are two possible solutions to this
problem?

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What will be the result of applying too much compound to a TLC plate?
You will run a TLC plate after the CC placing in one spot the original sample of pigments, one spot from the yellow band and one from the green band. Draw a picture of what you expect to observe after running the TLC plate. Explain.
Why are some of the detectors listed unsuitable for HPLC? Which of the GC detectors ) in the table below ) are suitable for HPLC?
How does increasing the polarity of the developing solvent affect the Rf of a compound on a silica gel TLC plate?
What will be the appearance of a TLC plate if a solvent of too low polarity is used for the development? too high polarity?
The solvent level in the development chamber should be above the level of the spotted compounds on the TLC plate. Group of answer choices True False
During TLC, what is the stationary phase and what is the mobile phase?
In what order would the following compounds be eluted on a silica gel TLC plate if they are spotted on the same plate and developed in a solvent mixture of 10:1 hexanes:ethyl acetate A. 1 has the highest Rf value, then 2 then 4 then 3 b. 4 has the highest Rf value, then 1 then 3 then 2 c. 3 has the highest Rf value, then 2 then 1 then 4 d. 2 has the highest Rf value, then 3 then 1 then 4
A3 A student performing the chromophores experiment noticed no pigments on their plate after developing the TLC plate. List 3 possible causes for this outcome and propose solutions for your given answers.
The three TLC plates below show the analysis of the same mixture of two compounds. One plate was developed in 100% DCM, one in 90:10 DCM/MeOH, and one in 80:20 DCM/MeOH. Determine which plate was developed in which solvent.
Although TLC is qualitative and tells you what is present in a given sample, it can also be used to determine where a compound might be lost: What samples could you test on a single TLC plate that might give you this information?
Which statement about TLC analyses is not true? * A- The line at the base of the TLC plate should be drawn with a pencil. B- To prevent contamination, a new capillary tube should be used to apply each sample. C- Samples should be applied multiple times in the same place to increase the concentrations of any possible compounds in the spot. D- A glass beaker lined with filter paper can be used as a development chamber in a TLC analysis. E- All these statements are correct.