zalez Name Cellular Respiration Background In this experiment, you will see that you exhale carbon dioxide. You will also determine what effect exercise and breathing into a bag has on the production of carbon dioxide, Carbon dioxide is produce when sugar and oxygen are combined during respiration: 6C02 +6H,0+Energy +60, 12 (Glucose) (0xygen) (ATPS) (Water) (Carbon dioxide) It is this process that provides our cells with the energy to move, blink, grow, breath, jump... By using a pH indicator, you can indirectly measure the amount of carbon dioxide dissolved. When carbon dioxide gas dissolves in water, the resulting carbonic acid decreases the pH of the solution. in this experiment, you will monitor the pH of 50 ml of a water solution (Na0H) by loolking for a color change. You will then see how breathing into a bag and exercising infuence carbonic acid formation. Н, СО, H.CO3 H2O (Water) C02 + (Carbonic acid) -(Carbon dioxide) Procedure: Relaxed 1. Select one member of your group. For consistency purposes, all tests should be done by the same student. Have this person relax in a chair. Juan Name of selected student 2. Place 50 ml of the NaOH solution in a 250 ml. Erlenmeyer flask. Have the selected student blow air through the straw and into the water. Keep blowing until the liquid is dear. You do not have to blow continuously. Several breaths may be necessary but keep the time betwen breaths at a minimum. 40 seconds Time to turn the liquid dear 2 Breathe into a bag 3. After the 'selected-one' has relaxed for a few minutes, have him/her breath normally into a bag for 45 seconds. Make sure the bag is sealed around the mouth and nose. 4. With the bag still. over his/her mouth, take a deep breath. 5. Have the subject exhale into the flask until the liquid is dlear. 28 Time to. turn the liquid dear seconds After exercise 5. After the selected student has relaxed, have him/her run/jog in place for 2 minutes. During this time, place 50 ml. of fresh NaOH solution into the flask. After the 2 minutes, have the subject breathe into the flask until the liquid is dear. 6. 21 seconds Time to turn the liquid dear Analysis of vata I. The initial solution is NaOH. Explain why this basic solution eventually becomes acidic.