A standard-addition plot should be created using the data given.
A commonform of standard-addition method involves adding known amounts of a standard solution to sample aliquots containing a fixed volume. Measurements taken for each solution can be used to plot a standard-addition plot.
Using the standard-addition plot constructed, the concentration of the unknown phenobarbital sample should be determined.
In a standard-addition plot, the graph can be extrapolated to obtain thevolume of standard reagentequivalent to the amount of analyte in the sample ( ). This value can be used in the following equation to calculate the concentration ( ) of the unknown sample.
= concentration of the standard
= constant volume of the unknown added to each sample solution.
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