The molar concentration of should be determined.
Calibration of instrumental methods: The purpose of calibration which is required by all types of analytical methods is quantitation. It is defined as a process in which the measured analytical signal and the concentration of analyte are related to each other. The three most common methods to calibrate are:
In case of standard addition method, standards are added to the aliquots of sample, keeping the matrix components same. On each solution, instrumental measurements are made to obtain instrument response ( S ).
The relation between the instrument response and concentration if they are proportional is,
represents the volume of sample.
represents the volume of standard.
represents the total volume of flask.
represents the concentration of standard.
represents the concentration of analyte in aliquot.
represents the proportionality constant.
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