Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 1, Problem 1CT
Summary Introduction

To determine:

The number of cells of Bacillus and yeast can be observed under a field of 2mm diameter.

Introduction:

Microscopy is a technique of observing an object under microscope that is not observable with naked eyes. The types of microscopy include, scanning probe microscopy, electron microscopy and optical microscopy. Optical microscopy uses light for illuminating and lenses to magnify the sample.

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If the diameter at the largest point of a cell covers, 20% of the field of view when observed at 400x magnification what would be the approximate diameter of the cell in micrometers??
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  • Which electron or light microscopy technique would you use for the following? (also explain)  For visualizing the fine structure of cellular organelles and cell architecture in the cell interior using very thin cross-sections made in Epon. To obtain sequential images of a macromolecule in order to study its activity in real-time. To obtain an extremely clear three-dimensional image of the nucleus of a single cell. What is the magnification of the image in a microscope using a 20x ocular and a 100x objective?
    Which of the following microscopes typically requires the use of vital dyes (like methylene blue) to visualize large subcellular structures in a living cell (like the nucleolus or the mitochondrion)? the scanning/tunneling electron microscope (STEM), with good resolution up to about 100,000,000x the transmission electron microscope (TEM), with good resolution up to about 100,000x the scanning electron microscope (SEM), with good resolution up to about 1,000,000x the compound light microscope, with good resolution up to about 1,500x all of the above microscopes would be equally useful in visualizing the interior of organelles
    Using an appropriate lens and oil immersion, what would be a microscope's maximum resolution (in micrometres) that could use a wavelength of 350 nm (assuming that such a device exists)? What would be the surface-to-volume ratio for the coccus-shaped bacterium twice the size of the maximum resolution you calculated? Why is the surface-to-volume ratio an important biological factor. Explain. Show all of your calculations.
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