Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 1, Problem 25P
Summary Introduction
To review:
The size of a DNA fragment affects its electrophoretic mobility on the electrophoresis gels.
Introduction:
Electrophoretic mobility is the migration of charged particles under the influence of applied electrical field. The cations move toward the negatively charged cathode, anions move toward the positively charged anode, and neutral species remain stationary.
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DNA fragments that are 500 bp, 1000 bp, and 2000 bp in length are separated by gel electrophoresis. Which fragment will migrate farthest in the gel?
a. The 2000-bp fragment
b. The 1000-bp fragment
c. The 500-bp fragment
d. All will migrate equal distances.
Variable number of tandem repeats (VNTRs) in the DNA molecule are highly useful in.......
A. Please briefly explain how Polymerase Chain Reaction works to amplify DNA.
B. Please briefly explain what gel electrophoresis is and how it works to separate a mixed sample of macromolecules like DNA.
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Chapter 1 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 1 - 1. Genetics affects many aspects of our lives....Ch. 1 - 2. How do you think the determination that DNA is...Ch. 1 - 3. A commentator once described genetics as “the...Ch. 1 - All life shares DNA as the hereditary material....Ch. 1 - Define the terms allele, chromosome, and gene and...Ch. 1 - 6. Define the terms genotype and phenotype, and...Ch. 1 - 7. Define natural selection, and describe how...Ch. 1 - Describe the modern synthesis of evolution, and...Ch. 1 - What are the four processes of evolution? Briefly...Ch. 1 - Define each of the following terms: a....
Ch. 1 - 11. Compare and contrast the genome, the proteome,...Ch. 1 - With respect to transcription describe the...Ch. 1 - Plant agriculture and animal domestication...Ch. 1 - Briefly describe the contribution each of the...Ch. 1 - If thymine makes up 21% of the DNA nucleotides in...Ch. 1 - What reactive chemical groups are found at the 5...Ch. 1 - Identify two differences in chemical composition...Ch. 1 - What is the central dogma of molecular biology?...Ch. 1 - A portion of a polypeptide contains the amino...Ch. 1 - The following segment of DNA is the template...Ch. 1 - 23. Fill in the missing nucleotides (so there are...Ch. 1 - 24. Suppose a genotype for a protein-producing...Ch. 1 - Prob. 25PCh. 1 - 26. Four nucleic acid samples are analyzed to...Ch. 1 - 27. What is meant by the term homology? How is...Ch. 1 - 28. If one is constructing a phylogeny of reptiles...Ch. 1 - 29. Consider the following segment of...Ch. 1 - 30. Ethical and social issues have become a large...Ch. 1 - 31. In certain cases, genetic testing can identify...Ch. 1 - 32. What information presented in this chapter and...Ch. 1 - 33. It is common to study the biology and genetics...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
Base analogs are mutagenic because of which characteristic?
a. They produce changes in DNA polymerase that cause it to malfunction.
b. They distort the structure of DNA.
c. They are similar in structure to the normal bases.
d. They chemically modify the normal bases.
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How would the results of this activity have been different if the DNA sequence you digested were circular instead of linear?
When restriction digests are performed and analyzed in the laboratory, one lane of the agarose gel is loaded with DNA ladders, which are DNA fragments of known sizes. What is the point of including these size standards?
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Suppose you have subjected the two given samples of EcoRl digested DNA to get electrophoresis. Draw a diagram of the expected gel to show the location of the cut DNA pieces. 
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the human immunodeficiency virus HIV uses RNA rather than DNA to encode genetic information. During infection, however, HIV uses an enzyme known as reverse transcriptase to generate double-stranded DNA. Generally speaking, how would the enzyme generate a double strand of DNA from a single strand of RNA?
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Genetics
Attached is a segment of DNA (doublestranded). Answer the following questions about the segment of DNA:
How many open reading frames (ORF) are in this sequence?
How many amino acids are encoded in all open reading frames in this segment/sequence?
Which strand is the template strand for the longest open reading frame?
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Which DNA repair systems you think might be capable of repairing a situation where T is in one strand and G is in the complementary strand? Explain dramatically.
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In experiments using polymerase chain reactions (PCR), it is often more difficult to amplifythrough regions of DNA that are high in GC content versus those regions that are either lower inGC content or are AT-rich. Based on your knowledge of DNA structure, explain why.
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What feature of a DNA fragment causes it to move through a gel during electrophoresis? a. the electrical charges of its phosphate groups b. its nucleotide sequence c. the hydrogen bonds between its base pairs d. its double helix shape
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A piece of linear DNA is 700000 base pairs in length and is digested with a restriction enzyme that recognizes a six base pairs site. How many fragments do you predict would be produced? Show all work.
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Describe the function of DNA polymerase. Explain why each part of the name DNA polymerase (DNA, polymer, -ase) makes sense.
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In the formation of recombinant DNA, a restriction endonuclease cuts a bacterial plasmid to give sticky ends. The DNA segments that are to be added to the plasmid are cleaved with the same restriction endonuclease. What aresticky ends and why is it important that the target DNA and the plasmid it will be incorporated into have complementary sticky ends?
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Okazaki fragments are short DNA pieces that explain how the DNA polymerase can continue the synthesis of the new strand.
True
False
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