Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
Question
Book Icon
Chapter 1, Problem 28P
Interpretation Introduction

(a)

Interpretation:

Whether MES, MOPS or HEPS is effective at pH 7.0 should be determined.

Concept introduction:

The effectiveness of a buffer can be determined by the fact that the pH values and the pKa lies close to each other. If this value is not close the buffer will not be effective.

Interpretation Introduction

(b)

Interpretation:

Whether MES, MOPS or HEPS is effective at pH 6.0 should be determined.

Concept introduction:

The effectiveness of a buffer can be determined by the fact that the pH values and the pKa lies close to each other. If this value is not close the buffer will not be effective.

Blurred answer
Students have asked these similar questions
What biochemical function do these Omega-3 fats play?  Much cheaper alternative sources of omega-3 fats include fats from some seeds and nuts (most often from flax seed in consumer products), but the allure of fish oil sources maintains these products' popularity despite much higher prices.  Is there a difference in the omega-3 fats derived from plant and animal sources?  If so, explain the difference and why one is "better" than the other.
Need help  Define Shannon Entropy. Provide an example using a short 10-mer of DNA.
Choice and Preparation of a Buffer System1. Choosing the proper buffer solution In Protein Precipitation, two liters of 5mM buffer solution with pH 5.2 is needed in the isolation of albumin. Which among the following buffer solution is best fitted for said purpose? Justify your answer.Buffer solutions                                                               pKa  Acetate buffer                                                                  4.73Tris- (hydroxymethy) aminomethane                                 8.08Phosphate buffer                                                               7.20 2. Preparation of the chosen buffer system Calculate and measure the amounts (in grams if solid and in mL if liquid) of weak acid and conjugate base needed to be able to prepare the chosen buffer system in part A above. Express your answer in useful units (that is, prepare it from practical amounts or concentrations of starting materials).
Knowledge Booster
Background pattern image
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
  • Text book image
    Biochemistry
    Biochemistry
    ISBN:9781305961135
    Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
    Publisher:Cengage Learning
    Text book image
    Biochemistry
    Biochemistry
    ISBN:9781305577206
    Author:Reginald H. Garrett, Charles M. Grisham
    Publisher:Cengage Learning
Text book image
Biochemistry
Biochemistry
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Cengage Learning
Text book image
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning