Chapter 1, Problem 2E.1RQ

(a)

Summary Introduction

To explain: The purpose of flaming inoculating instrument prior to and after each inoculation.

Introduction: Subculture technique is used to carry on the growth of the bacteria. During subculture, a bacterium is transferred from the initial growth medium to another medium, which is useful for microbial tests and also in producing bulk stock cultures of the desired bacterium.

(b)

Summary Introduction

To explain: The purpose of holding the test tube caps in the hand.

Introduction: Subculture technique is used to carry on the growth of the bacteria. During the subculture, a bacterium is transferred from the initial growth medium to another medium, which is useful for microbial tests and also in producing bulk stock cultures of the desired bacterium.

(c)

Summary Introduction

To explain: The importance of cooling the inoculating instrument prior to obtaining the inoculum.

Introduction: Subculture technique is used to carry on the growth of the bacteria. During the subculture, a bacterium is transferred from the initial growth medium to another medium, which is useful for microbial tests and also in producing bulk stock cultures of the desired bacterium.

(d)

Summary Introduction

To explain: The importance of flaming the neck of the tubes immediately after uncapping and before recapping.

Introduction: Subculture technique is used to carry on the growth of the bacteria. During the subculture, a bacterium is transferred from the initial growth medium to another medium, which is useful for microbial tests and also in producing bulk stock cultures of the desired bacterium.