Concept explainers
To review:
The results of fusion of a promoter for an SOS response gene with the luciferase open reading frame.
Introduction:
The class of oxidative enzymes that produces bioluminescence is defined as luciferase. They have wide applications in the field of
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The reporter genes are
It can be used as a real-time biosensor for environmental stress response. RecA (recombination A) protein that is stimulated by the single-stranded DNA (deoxyribonucleic acid) is involved in the inactivation of the repressor (LexA) of SOS response genes, which induce the response. The fusion of the luciferase gene with a recA promoter will show an increase in the fluorescence after the irradiation by ultraviolet light by putting the culture into a spectrophotometer.
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Microbiology: An Evolving Science (Fourth Edition)
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