GEN COMBO LOOSELEAF BIOLOGY:CONCEPTS & INVESTIGATIONS; CONNECT ACCESS CARD
GEN COMBO LOOSELEAF BIOLOGY:CONCEPTS & INVESTIGATIONS; CONNECT ACCESS CARD
4th Edition
ISBN: 9781260149289
Author: Hoefnagels
Publisher: MCG
bartleby

Concept explainers

bartleby

Videos

Textbook Question
Chapter 11, Problem 1MCQ

If a restriction enzyme cuts between the G and the A whenever it encounters the sequence GAATTC, how many fragments will be produced when the enzyme is digested with DNA with the following sequence? TGAGAATTCAACTGAATTCAAATTCGAATTCTTAGC

  1. a. Two
  2. b. Three
  3. c. Four
  4. d. Five
Expert Solution & Answer
Check Mark
Summary Introduction

Introduction:

Restriction enzymes are the enzymes that digest the higher molecular weight DNA in to smaller fragments. They are also known as restriction endonucleases.

Answer to Problem 1MCQ

Correct answer:

There are three sequences of GAATTC present on the DNA fragment and the fragments formed after the cleavage of the DNA are four in numbers. Therefore, option (c) is correct.

Explanation of Solution

Reason for correct statement:

Restriction endonucleases digest a double stranded DNA at specific sites. These specific sites are also called recognition site that present as palindrome. If the restriction enzyme given cuts between the G and A whenever it encounters the sequence GAATTC on the given DNA with the following sequence TGAGAATTCTGAATTCAAATTCGAATTCTTAGC, the cleaving of the DNA will be given as follows:

GEN COMBO LOOSELEAF BIOLOGY:CONCEPTS & INVESTIGATIONS; CONNECT ACCESS CARD, Chapter 11, Problem 1MCQ

As shown, after the cleavage of the DNA by the help of the restriction enzyme, four fragments will be formed.

Option (c) is given as “Four”.

As, “the restriction enzyme recognizes three sequence on the given DNA, so it will make three cuts, that results in the production of the four DNA fragments,” is the right answer.

Hence, option (c) is correct.

Reasons for the incorrect statements:

Option (a), is given as “Two”.

The fragments of the DNA formed will be four after the action of the restriction enzyme. Hence, it is a wrong answer.

Option (b), is given as “Three”.

The DNA fragment formed after the cleavage will be four in numbers. Hence, it is a wrong answer.

Option (d), is given as “Five”.

For DNA fragments will be formed after the cutting action of the restriction enzyme at the given recognition site. Hence, it is a wrong answer.

Hence, options (a), (b), and (d), are incorrect.

Conclusion

Restriction enzymes are also known as molecular scissors that could cut double stranded DNA molecules at specific sites. It is an important tool that is used in the manipulation of DNA.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!
Students have asked these similar questions
a) what are restriction enzymes? b) What is the main function of restriction enzymes in nature? c) Compare and contrast the these enzymes in nature and in scientific research.
What do genetic engineers use to create the “sticky ends” needed to splice two fragments of DNA together? a.) an amino acid sequence b.) DNA ligase c.) restriction enzymes d.) mRNA
The function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b. separate the DNA double helix c. cut the nucleotide sequence at a specific location in DNA d. proofread DNA for accidental damages and corrects these errors

Chapter 11 Solutions

GEN COMBO LOOSELEAF BIOLOGY:CONCEPTS & INVESTIGATIONS; CONNECT ACCESS CARD

Knowledge Booster
Biology
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
    • SEE MORE QUESTIONS
    Recommended textbooks for you
  • Human Heredity: Principles and Issues (MindTap Co...
    Biology
    ISBN:9781305251052
    Author:Michael Cummings
    Publisher:Cengage Learning
    Concepts of Biology
    Biology
    ISBN:9781938168116
    Author:Samantha Fowler, Rebecca Roush, James Wise
    Publisher:OpenStax College
  • Human Heredity: Principles and Issues (MindTap Co...
    Biology
    ISBN:9781305251052
    Author:Michael Cummings
    Publisher:Cengage Learning
    Concepts of Biology
    Biology
    ISBN:9781938168116
    Author:Samantha Fowler, Rebecca Roush, James Wise
    Publisher:OpenStax College
    Molecular Techniques: Basic Concepts; Author: Dr. A's Clinical Lab Videos;https://www.youtube.com/watch?v=7HFHZy8h6z0;License: Standard Youtube License