PRINCIPLES OF LIFE
PRINCIPLES OF LIFE
3rd Edition
ISBN: 9781319017712
Author: HILLIS
Publisher: MAC HIGHER
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Chapter 12, Problem 1.1Q
Summary Introduction

To differentiate: The Sanger sequencing and high throughput sequencing.

Introduction: The genome of an organism is defined as the whole genetic information that is inherited from one generation to the next generation. It includes the protein-coding genes, non-protein coding genes, regulatory genes, chloroplast DNA and mitochondrial DNA. The human genome consists of about 2.5 billion gens of which only 20,000 genes are protein-coding genes.

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Explanation of Solution

The difference between the Sanger sequencing and high throughput sequencing is described as follows:

CharacteristicsSanger sequencingHigh throughput sequencing
  • Template preparation
  • The direct DNA template is used.
  • The PCR technique is used to amplify the template.
  • Sequencing chemistry
  • Chain termination
  • Sequencing by amplification.
  • Fluoresce tags.
  • The fluoresce is tagged with deoxyribonucleotides.
  • The fluoresce is tagged with deoxyribonucleotides.
  • Rounds
  • It involves a single round of sequencing.
  • It involves multiple rounds of sequencing.
  • Number of base pairs.
  • It can sequence about 300 to 1000 bases.
  • It can sequence about a million bases.

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