Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Question
Chapter 12.1, Problem 1TQ
Summary Introduction
To review:
The modification of mutagenesis with transposons to identify mutants defective in the synthesis of amino acid.
Introduction:
Transposons are the segments of DNA (deoxyribonucleic acid) that can change its site within the genome. They can also alter the genetic information possessed within the genome. This property of transposons can be used to transfer the foreign gene into the host. The process by which the genes are transferred within the chromosome of the host organism using transposons is called transposon mutagenesis. This leads to modification or interruption in the function of the original gene.
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Why are regulatory mutants used to increase the production of glutamic acid by Corynebacterium glutamicum?
Given the following phenotypes, explain how the mutation (identified by a (-) superscript) will affect the E. coli grown in lactose medium.
a. l+p+o+z-y+
b. i-p+o+z+y+
c. l+p+o-z+y+
d. l+p-o+z+y+
How can the foreign protein be modified to facilitate its purification?
Chapter 12 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 12.1 - Prob. 1TQCh. 12.1 - Prob. 2TQCh. 12.1 - Prob. 3TQCh. 12.1 - Prob. 4TQCh. 12.2 - Prob. 1TQCh. 12.2 - Prob. 2TQCh. 12.2 - Prob. 3TQCh. 12.3 - Prob. 1TQCh. 12.4 - Prob. 1TQCh. 12.4 - Prob. 2TQ
Ch. 12.5 - Prob. 1TQCh. 12 - Prob. 1RQCh. 12 - Prob. 2RQCh. 12 - Prob. 3RQCh. 12 - Prob. 4RQCh. 12 - Prob. 5RQCh. 12 - Prob. 6RQCh. 12 - Prob. 7RQCh. 12 - Prob. 8RQCh. 12 - Prob. 9RQCh. 12 - Prob. 10RQCh. 12 - Prob. 11RQCh. 12 - Prob. 12RQCh. 12 - Prob. 13RQCh. 12 - Prob. 14RQCh. 12 - Prob. 15RQCh. 12 - Prob. 1TQCh. 12 - Prob. 2TQCh. 12 - Prob. 3TQ
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