Biology
Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 12.2, Problem 1CS

Core Skill: Connections Look back at the role of DNA polymerase shown in Figure 11.15. What are similarities and differences between the function of DNA polymerase and that of RNA polymerase?

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VISUAL SKILLS Compare Figure 20.7 with Figure 16.20.How does replication of DNA ends during PCR proceedwithout shortening the fragments each time?
PCR Review: How are you able to target specific genes in PCR? What were some possible sources of error during your DNA Extraction and PCR experiments?
Please help witht this homework question A- What are the topological parameters (linking number, twist, and writhe)for a relaxed, 4,200 base pair circular double-stranded DNA plasmid? LK=?   Tw= ?   Wr=? The CRISPR-Cas9 protein first forms a protein-RNA complex with a guide RNA, and then binds to DNA sequences that match a 20-base target sequence within the guide. When the CRISPR-Cas9 complex binds to DNA, it unwinds about 20 base pairs of the DNA double helix. B- Why is DNA unwinding required for CRISPR-Cas9 protein-RNA complexto recognize target sites? C-How would the topological parameters (linking number, twist, andwrithe) of the 4,200 bp plasmid in (A) change when the CRISPR-Cas9 complex binds the DNA and unwinds about 20 bp? Lk=?  Tw=?  Wr=?  D- What is the linking number after the CRISPR-Cas9 complex cleaves theDNA?
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