Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 12.2, Problem 1TQ
Summary Introduction
To review:
The result of the northern blot and western blot of the gad genes.
Introduction:
Classic molecular biology techniques are used to examine a specific gene or a protein. These techniques include northern blotting, southern blotting, and the western blotting used for the examination of RNA (ribonucleic acid), DNA (deoxyribonucleic acid), and the protein, respectively. The basic principle of separation in all the techniques is gel electrophoresis.
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For the experiment shown Fig. 6.30 and 6.31, what is the advantage to use a labeled incoming nucleotide (α-32P-UTP or α-32P-CTP), not labeled reagent 1 itself? Please draw a diagram to help explain your answer by contrasting the RNA polymerase labeled with α-32P-Reagent (Panel A) vs α-32P-UTP (Panel B).
Design a six-residue nucleic acid probe that would hybridize with the greatest number of E. coli gene promoters.
How might such a “competition assay” be used to determine the biological glycosaminoglycan that binds to protein E?
Chapter 12 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 12.1 - Prob. 1TQCh. 12.1 - Prob. 2TQCh. 12.1 - Prob. 3TQCh. 12.1 - Prob. 4TQCh. 12.2 - Prob. 1TQCh. 12.2 - Prob. 2TQCh. 12.2 - Prob. 3TQCh. 12.3 - Prob. 1TQCh. 12.4 - Prob. 1TQCh. 12.4 - Prob. 2TQ
Ch. 12.5 - Prob. 1TQCh. 12 - Prob. 1RQCh. 12 - Prob. 2RQCh. 12 - Prob. 3RQCh. 12 - Prob. 4RQCh. 12 - Prob. 5RQCh. 12 - Prob. 6RQCh. 12 - Prob. 7RQCh. 12 - Prob. 8RQCh. 12 - Prob. 9RQCh. 12 - Prob. 10RQCh. 12 - Prob. 11RQCh. 12 - Prob. 12RQCh. 12 - Prob. 13RQCh. 12 - Prob. 14RQCh. 12 - Prob. 15RQCh. 12 - Prob. 1TQCh. 12 - Prob. 2TQCh. 12 - Prob. 3TQ
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- A graduate student studying the pathogenic bacteria Acinetobacter baumannii made cDNA from planktonic cells and biofilm cells and performed RNA-Seq on both samples. She aligned her sequencing reads to a locus of the baumannii genome as shown. a. Which genes are on an operon together? Explain which data supports this? b. What is the most expressed transcript from the locus in Planktonic culture? c. Order the transcripts from largest increase in relative expression between biofilm and planktonic cells to the largest decrease in relative expression. d. When this data was compared to microarray transcriptional profiling, the microarray data provided lower expression levels for the most highly expressed transcripts. Why did this occur?arrow_forwardHow can the binding assay approach be utilized to match coding triplets with their amino acid counterparts?arrow_forwardFor the experiment outlined in Figure 15.8, could Nirenberg and Matthaei have substituted RNA polymerase instead of polynucleotide phosphorylase without otherwise modifying the experiment? Why or why not?arrow_forward
- Refer to the DNA sequence provided: 3’ -TACTGAAGCGGCAGCCCCGCATGAGTAGACCTTACT-5’ a. What is the mRNA transcript of the anticoding strand of the DNA model? b. What is the amino acid sequence of the polypeptide chain that will be translated from the mRNA in (a)?arrow_forwardHow would you modify the Ames test to evaluate physical mutagens?Would it be necessary to add the rat liver extract? Explain why or why not.arrow_forwardYou ran the agarose gel but forgot to include the lactose-induced 23S reaction (template - cDNA). Without repeating the gel, what conclusions could you make about gst::egfp mRNA levels after lactose and IPTG induction? What would remain inconclusive? What if the sample you forgot to include was the negative control of lactose-induced 23S reaction (template - RNA)?arrow_forward
- What would be the phenotype of an E. coli strain in which the tandem tryptophan codons in the leader region were mutated so that they coded for serine instead?arrow_forwardDescribe a ChIP-Seq experiment that will enable the identification of promoters to which the stationary phase sigma factor RpoS binds in E. coli. How could you also determine when RpoS binds to DNA?arrow_forwardusing example what is a degenerate primer? with the aid of diagrams discuss how degenerate primers are used to introduce random mutations in a gene and list some advantages and disadvantages of generating modified protein by this method .arrow_forward
- Describe the steps, starting from an endonuclease digested DNA sample, to complete a Southern blot experiment. What difference would a Northern blot have?arrow_forwardDescribe how an electrophoretic mobility shift assay is used to determine if a protein binds to DNA or RNA.arrow_forwardEhat primer sets could be amplify the following DNA sequence? AATACGTCGCATGGggatccttttttatgcatgarrow_forward
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