The reason for the preference of sticky ends over blunt ends generated by restriction endonucleases. Introduction: Restriction enzymes are also known as molecular scissors. These cut the DNA fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans , and Smith . Some of the examples of restriction enzymes are Hind III, EcoRI, BamHI, HpaI, and SmaI.

Question

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Answer 1

Question

Chapter 12.4, Problem 11SAQ

Summary Introduction

To explain:

The reason for the preference of sticky ends over blunt ends generated by restriction endonucleases.

Introduction:

Restriction enzymes are also known as molecular scissors. These cut the DNA fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans, and Smith. Some of the examples of restriction enzymes are Hind III, EcoRI, BamHI, HpaI, and SmaI.

Expert Solution & Answer

Explanation of Solution

Pictorial representation:

Biology: How Life Works, Chapter 12.4, Problem 11SAQ

The restriction fragments produced by restriction enzymes can either have blunt ends or sticky ends. Sticky ends generated by restriction enzymes like EcoRI, HindIII, and BamHI are of great value as they can be attached easily with other sticky ends. The sticky end produced by one restriction enzyme can only attach with the sticky end produced by that restriction enzyme only. However, blunt end of one restriction enzyme can attach to any other blunt end.

Conclusion

Sticky ends produced by one restriction enzyme are preferred because they can easily ligate with other fragments of the DNA.

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Answer 2

Question

Chapter 12.4, Problem 11SAQ

Summary Introduction

To explain:

The reason for the preference of sticky ends over blunt ends generated by restriction endonucleases.

Introduction:

Restriction enzymes are also known as molecular scissors. These cut the DNA fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans, and Smith. Some of the examples of restriction enzymes are Hind III, EcoRI, BamHI, HpaI, and SmaI.

Expert Solution & Answer

Explanation of Solution

Pictorial representation:

Biology: How Life Works, Chapter 12.4, Problem 11SAQ

The restriction fragments produced by restriction enzymes can either have blunt ends or sticky ends. Sticky ends generated by restriction enzymes like EcoRI, HindIII, and BamHI are of great value as they can be attached easily with other sticky ends. The sticky end produced by one restriction enzyme can only attach with the sticky end produced by that restriction enzyme only. However, blunt end of one restriction enzyme can attach to any other blunt end.

Conclusion

Sticky ends produced by one restriction enzyme are preferred because they can easily ligate with other fragments of the DNA.

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Answer 3

Question

Chapter 12.4, Problem 11SAQ

Summary Introduction

To explain:

The reason for the preference of sticky ends over blunt ends generated by restriction endonucleases.

Introduction:

Restriction enzymes are also known as molecular scissors. These cut the DNA fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans, and Smith. Some of the examples of restriction enzymes are Hind III, EcoRI, BamHI, HpaI, and SmaI.

Expert Solution & Answer

Explanation of Solution

Pictorial representation:

Biology: How Life Works, Chapter 12.4, Problem 11SAQ

The restriction fragments produced by restriction enzymes can either have blunt ends or sticky ends. Sticky ends generated by restriction enzymes like EcoRI, HindIII, and BamHI are of great value as they can be attached easily with other sticky ends. The sticky end produced by one restriction enzyme can only attach with the sticky end produced by that restriction enzyme only. However, blunt end of one restriction enzyme can attach to any other blunt end.

Conclusion

Sticky ends produced by one restriction enzyme are preferred because they can easily ligate with other fragments of the DNA.

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Answer 4

Question

Chapter 12.4, Problem 11SAQ

Summary Introduction

To explain:

The reason for the preference of sticky ends over blunt ends generated by restriction endonucleases.

Introduction:

Restriction enzymes are also known as molecular scissors. These cut the DNA fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans, and Smith. Some of the examples of restriction enzymes are Hind III, EcoRI, BamHI, HpaI, and SmaI.

Expert Solution & Answer

Explanation of Solution

Pictorial representation:

Biology: How Life Works, Chapter 12.4, Problem 11SAQ

The restriction fragments produced by restriction enzymes can either have blunt ends or sticky ends. Sticky ends generated by restriction enzymes like EcoRI, HindIII, and BamHI are of great value as they can be attached easily with other sticky ends. The sticky end produced by one restriction enzyme can only attach with the sticky end produced by that restriction enzyme only. However, blunt end of one restriction enzyme can attach to any other blunt end.

Conclusion

Sticky ends produced by one restriction enzyme are preferred because they can easily ligate with other fragments of the DNA.

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Answer 5

Chapter 12.4, Problem 11SAQ

Summary Introduction

To explain:

The reason for the preference of sticky ends over blunt ends generated by restriction endonucleases.

Introduction:

Restriction enzymes are also known as molecular scissors. These cut the DNA fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans, and Smith. Some of the examples of restriction enzymes are Hind III, EcoRI, BamHI, HpaI, and SmaI.

Expert Solution & Answer

Explanation of Solution

Pictorial representation:

Biology: How Life Works, Chapter 12.4, Problem 11SAQ

The restriction fragments produced by restriction enzymes can either have blunt ends or sticky ends. Sticky ends generated by restriction enzymes like EcoRI, HindIII, and BamHI are of great value as they can be attached easily with other sticky ends. The sticky end produced by one restriction enzyme can only attach with the sticky end produced by that restriction enzyme only. However, blunt end of one restriction enzyme can attach to any other blunt end.

Conclusion

Sticky ends produced by one restriction enzyme are preferred because they can easily ligate with other fragments of the DNA.

Answer 6

Chapter 12.4, Problem 11SAQ

Summary Introduction

To explain:

The reason for the preference of sticky ends over blunt ends generated by restriction endonucleases.

Introduction:

Restriction enzymes are also known as molecular scissors. These cut the DNA fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans, and Smith. Some of the examples of restriction enzymes are Hind III, EcoRI, BamHI, HpaI, and SmaI.

Expert Solution & Answer

Explanation of Solution

Pictorial representation:

Biology: How Life Works, Chapter 12.4, Problem 11SAQ

The restriction fragments produced by restriction enzymes can either have blunt ends or sticky ends. Sticky ends generated by restriction enzymes like EcoRI, HindIII, and BamHI are of great value as they can be attached easily with other sticky ends. The sticky end produced by one restriction enzyme can only attach with the sticky end produced by that restriction enzyme only. However, blunt end of one restriction enzyme can attach to any other blunt end.

Conclusion

Sticky ends produced by one restriction enzyme are preferred because they can easily ligate with other fragments of the DNA.

Answer 7

Chapter 12.4, Problem 11SAQ

Summary Introduction

To explain:

The reason for the preference of sticky ends over blunt ends generated by restriction endonucleases.

Introduction:

Restriction enzymes are also known as molecular scissors. These cut the DNA fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans, and Smith. Some of the examples of restriction enzymes are Hind III, EcoRI, BamHI, HpaI, and SmaI.

Answer 8

Expert Solution & Answer

Explanation of Solution

Pictorial representation:

Biology: How Life Works, Chapter 12.4, Problem 11SAQ

The restriction fragments produced by restriction enzymes can either have blunt ends or sticky ends. Sticky ends generated by restriction enzymes like EcoRI, HindIII, and BamHI are of great value as they can be attached easily with other sticky ends. The sticky end produced by one restriction enzyme can only attach with the sticky end produced by that restriction enzyme only. However, blunt end of one restriction enzyme can attach to any other blunt end.