Brock Biology of Microorganisms (15th Edition)

15th Edition

ISBN: 9780134261928

Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl

Publisher: PEARSON

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Textbook Question

Chapter 12.4, Problem 1MQ

How can site-directed mutagenesis be useful to enzymologists?

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Chapter 12.3, Problem 1CR

Chapter 12.4, Problem 2MQ

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Chapter 12 Solutions

Brock Biology of Microorganisms (15th Edition)

Ch. 12.1 - Why is a primer needed at each end of the DNA...Ch. 12.1 - How does RT-PCR differ from traditional PCR?Ch. 12.1 - Prob. 3MQ Ch. 12.1 - Describe the basic principles of gene...Ch. 12.2 - What is the purpose of molecular cloning?Ch. 12.2 - Prob. 2MQ Ch. 12.2 - Prob. 3MQ Ch. 12.2 - Prob. 1CR Ch. 12.3 - How can the bacteriophage T7 promoter be used to...Ch. 12.3 - What major advantage does cloning mammalian genes...

Ch. 12.3 - Prob. 3MQ Ch. 12.3 - Prob. 1CR Ch. 12.4 - How can site-directed mutagenesis be useful to...Ch. 12.4 - What is used to alter more than a few base pairs...Ch. 12.4 - What are knockout mutations?Ch. 12.4 - What does site-directed mutagenesis allow you to...Ch. 12.5 - What is a reporter gene? The product of which...Ch. 12.5 - Prob. 2MQ Ch. 12.5 - Describe two widely used reporter genes.Ch. 12.6 - Prob. 1MQ Ch. 12.6 - Prob. 2MQ Ch. 12.6 - Prob. 3MQ Ch. 12.6 - Prob. 1CR Ch. 12.7 - Prob. 1MQ Ch. 12.7 - Give an example of a genetically modified plant...Ch. 12.7 - How have transgenic salmon been engineered to...Ch. 12.7 - What is the Ti plasmid and how has it been of use...Ch. 12.8 - Explain why recombinant vaccines might be safer...Ch. 12.8 - Prob. 2MQ Ch. 12.8 - Prob. 3MQ Ch. 12.8 - What is a subunit vaccine and why are subunit...Ch. 12.9 - Explain why metagenomic cloning gives large...Ch. 12.9 - What types of environments are often sampled to...Ch. 12.9 - Prob. 3MQ Ch. 12.9 - How has metagenomics been used to find novel...Ch. 12.10 - How has Caldicellulosiruptor been modified to...Ch. 12.10 - Prob. 2MQ Ch. 12.10 - What has been the limiting factor in engineering...Ch. 12.10 - Prob. 1CR Ch. 12.11 - What are biobricks?Ch. 12.11 - Prob. 2MQ Ch. 12.11 - How was Escherichia coli modified to produce a...Ch. 12.11 - Prob. 1CR Ch. 12.12 - Prob. 1MQ Ch. 12.12 - Prob. 2MQ Ch. 12.12 - How is recombinant DNA inserted into a genome...Ch. 12.12 - How has the CRISPR editing technology been applied...Ch. 12.13 - Prob. 1MQ Ch. 12.13 - How can a tRNA be engineered to encode for a...Ch. 12.13 - Prob. 3MQ Ch. 12.13 - What are some mechanisms for controlling a...Ch. 12 - Suppose you have just determined the DNA base...Ch. 12 - Prob. 2AQ Ch. 12 - Prob. 3AQ Ch. 12 - Describe how you could recode Escherichia coli to...

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How can site-directed mutagenesis be useful to enzymologists?

Solution Summary: The author explains that site-directed mutagenesis induces mutations in genes or DNA at precisely determined sites using synthetic DNA and DNA cloning techniques.

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