The calibration curve between absorbance and concentration of standard series should be plotted. Using the regression line & the plot the concentration of the unknown solution should be calculated. The accuracy of these 2 methods should be compared by considering the final answers.Error analysis of the data should be done.
The absorbance of light can be determined by the Beer-Lambert law. The chemical equation relevant to the law is,
= Concentration of the solution
= Path length of the cell
= Absorptivity of the solution
Standard deviation of a data series can be calculated by the following equation.
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