(a)
Interpretation:
The absorbance of the solution containing the complex formed between
Concept introduction:
The absorbance of the solution is the ability of the solution to absorb the monochromatic light passing through it. The absorbance of the solution is defined as the ratio of the intensity of light incident on the solution to the intensity of light absorbed by the solution.
The relation between the absorbance, path length, molar absorptivity and the concentration of the solution is given as.
(b)
Interpretation:
The percent transmittance of the solution formed from the complex between
Concept introduction:
The percent transmittance of a solution is the ability of the solution to allow the monochromatic light to pass through it. The percent transmittance of a solution is the ratio of the intensity of monochromatic light incident on the solution to the intensity transmitted through the solution.
The relation between the absorbance and transmittance is established by the Beer’s law.
(c)
Interpretation:
The concentration of the solution is to be determined for the change in the path length of the cell containing the solution.
Concept introduction:
The concentration of the solution, path length of the cell and the molar absorptivity of the solution plays an important role in determining the absorbance of the solution. If any of the parameter is affected, it leads to change in the absorbance of the solution.
The relation between the absorbance, path length, molar absorptivity and the concentration of the solution is given as.
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Chapter 13 Solutions
Principles of Instrumental Analysis
- The molar absorptivity (ε) of the FeSCN2+ complex ion is 4700 M-1 · cm-1 at a wavelength of 450 nm. Using a 1-cm sample tube, you measure the absorbance as (2.0x10-1). What is the concentration of FeSCN2+?arrow_forwardAt 580 nm, the wavelength of its maximum absorption, the complex Fe(SCN)2+ has a molar absorptivity of 7.00x103 L mol-1 cm-1. Calculate a.) the absorbance of 2.50x10-5 M solution of the complex at 580 nm in 1.00-cm cell. b.) the absorbance of a solution in which the concentration of the complex is twice that in part (a). c.) the transmittance of the solutions described in parts (a) and (b). d.) the absorbance of a solution that has half the transmittance of that described in part (a).arrow_forwardAt 580 nm, the wavelength of its maximum absorption, the complex Fe(SCN)2+ has a molar absorptivity of 7.00x 103 L cm-1 mol-1. Calculate (a) the absorbance of a 3.40 x 10–5 M solution of the complex at 580 nm in a 1.00-cm cell. (b) the absorbance of a solution in which the concentration of the complex is twice that in (a). (c) the transmittance of the solutions described in (a) and (b). (d) the absorbance of a solution that has half the transmittance of that described in (a).arrow_forward
- The absorption coefficient of a glycogen-iodine complex is 0.20 at light of 450 nm. What is the concentration when the transmission is 40 % in a cuvette of 2 cm?arrow_forwardMercury(II) forms a 1:1 complex with triphenyltetrazolium chloride that exhibits an absorption maximum at 255 nm. The mercury(II) in a soil sample was extracted into an organic solvent containing an excess of TTC , and the resulting solution was diluted to 100.0 mL in a volumetric flask. Five-milliliter aliquots of the analyte solution were then transferred to six 25-mL volumetric flasks. A standard solution was then prepared that was 5*10^-6 M in . Volumes of the standard solution shown in the table were then pipetted into the volumetric flasks, and each solution was then diluted to 25.00 mL. The absorbance of each solution was measured at 255 nm in 1.00-cm quartz cells. Please solve and explain these questions: A.) Enter the given data pictured above into a spreadsheet and show the correct standard additions plot B.) Determine the slope and the intercept of the line C.)Determine the standard deviation of the slope and the intercept. D.) Calculate the concentration of Hg(II) in the…arrow_forwardCalculate the molar absorptivity and absorbance of a 1.00 x 104 M solution, which has atransmittance of 32.1% , when the path length is 2.5 cm at 650 nm. (arrow_forward
- The molar absorptivity for the FD&C dye “Red No. 3” (Erythrosine) 8.25104 M‒1·cm‒1 at 526 nm. Calculate the expected absorbance of solution of Red No. 3 with a concentration of 4.00×10–6M when measured at 629 nm in a 2.00 cm cell.arrow_forwardA metal complex with a Molecular mass of 820 g mol−1 was dissolved and the solution made up to 25 mL in a volumetric flask. 1 mL was withdrawn, placed in a 100 mL volumetric flask and diluted to the mark. The absorbance (at 457 nm) of this solution (in a 1 cm cuvette) was 0.245. The extinction coefficient for this compound at 457 nm (ε530) = 11715 M-1cm-1.(a) calculate the concentration of the compound in the cuvette.(b) calculate the concentration of the compound in the 25mL volumetric flask(c) determine the mass of compound used to make the 25mL solutionarrow_forwardThe molar absorptivity for the complex formed between bismuth(III) and thiourea is 9.32x103 L cm-1 mol-1 at 470 nm. Calculate the concentration (in mol/L) of the complex if the absorbance is 0.845 measured with a 1.00 cm cell.arrow_forward
- At 580 nm, the wavelength of its maximum absorption, the complex Fe(SCN)2+ has a molar absorptivity of 7.00x103 L mol-1 cm-1. Calculate letter D ONLY a.) the absorbance of 2.50x10-5 M solution of the complex at 580 nm in 1.00-cm cell. = 0.175 b.) the absorbance of a solution in which the concentration of the complex is twice that in part (a). c.) the transmittance of the solutions described in parts (a) and (b). d.) the absorbance of a solution that has half the transmittance of that described in part (a).arrow_forwardA solution of compound Z absorbs light of wavelength 256 nm, and for this absorption, log ε=3.3. What is the concentration of a solution of Z (in an optical cell of path length 1 cm) that gives the absorbance reading is 0.21?arrow_forwardThe ultraviolet spectrum of aspirin shows a secondary absorption band at 271 nm. If a solution of aspirin in water, with a concentration of 1×10-4 molar solution is examined at 271 nm, what will be the absorbance reading (ℇ = 1000) and what will be the intensity ratio, IO/I, respectively?arrow_forward
- Principles of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning