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Biochemistry

9th Edition
Mary K. Campbell + 2 others
ISBN: 9781305961135

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Section
BuyFindarrow_forward

Biochemistry

9th Edition
Mary K. Campbell + 2 others
ISBN: 9781305961135
Textbook Problem

RECALL What would be an advantage of using HaeIII for a cloning experiment? What would be a disadvantage?

Interpretation Introduction

Interpretation:

The advantage and disadvantage of using HaeIII in the cloning process are to be determined.

Concept introduction:

DNA fragments are separated by using gel electrophoresis.

DNA is fragmented by using restriction endonucleases.

Endonucleases usually cut between a palindromic sequence which seems to be the same from left to right as well as from right to left.

Explanation

Endonucleases usually cut between a palindromic sequence that seems to be the same from left to right as well as from right to left. The main advantage of HaeIII is that it provides the blunt ends. The sequences cut by HaeIII are usually of four bases. The cut is in the middle of the sequence. Unsharpened ends are left by it

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