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Human Heredity: Principles and Iss...

11th Edition
Michael Cummings
Publisher: Cengage Learning
ISBN: 9781305251052

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BuyFindarrow_forward

Human Heredity: Principles and Iss...

11th Edition
Michael Cummings
Publisher: Cengage Learning
ISBN: 9781305251052
Chapter 13, Problem 14QP
Textbook Problem
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Cloned Libraries

You are given the task of preparing a cloned genomic library from a human tissue culture cell line. What type of vector would you select for this library and why?

Summary Introduction

To describe: The type of vector used in the preparation of a cloned genomic library from human tissue culture cell line.

Introduction: In the manufacturing of human genomic library, the fragments of DNA used in the process can vary in size which can be picked up by the plasmid easily. About 8 million plasmids are required to include all the genetic information from an individual cell.

Explanation of Solution

The vector that can be used in the preparation of a cloned genomic library is yeast artificial chromosome (YAC). The YAC vectors are derived from the eukaryotic yeast cells. They have centromeres and telomeres in their structure that are capable to hold sequences of long lengths...

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