Concept explainers
To describe: The reasons behind the revolutionary concept of PCR.
Introduction: A process of molecular biology which is responsible for the production of various copies of a particular segment of DNA is called “polymerase chain reaction (PCR).” Using the technique of PCR, a single segment of DNA can be amplified to produce a sufficient number of copies of that particular segment.
To describe: The two applications of PCR.
Introduction: A process of molecular biology which is responsible for the production of various copies of a particular segment of DNA is called “polymerase chain reaction (PCR).” Using the technique of PCR, a single segment of DNA can be amplified to produce a sufficient number of copies of that particular segment.
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Chapter 13 Solutions
Human Heredity: Principles and Issues (MindTap Course List)
- Cloned Libraries You are running a PCR to generate copies of a fragment of the cystic fibrosis (CF) gene. Beginning with two copies at the start, how much of an amplification of this fragment will be present after six cycles in the PCR machine?arrow_forwardAnalyzing Cloned Sequences What kind of information can a DNA sequence provide to a researcher studying a disease-causing gene?arrow_forwardWhat is the difference between PCR and real time PCR? and What happens during PCR? Write in detail.arrow_forward
- Briefly explain the rationales of adding chemicals that can affect DNA stability in a polymerase chain reaction (PCR). Why DNA melting is required in PCR? Briefly explain how PCR can be used to detect DNA mutation.arrow_forwardWhat are the different types of PCR and their product concepts? Site and explain the comparison briefly.arrow_forwardIn PCR, the goal is to make copies of?arrow_forward
- PCR is a very useful method in biotechnology because it does not require the 6 or more different key proteins/enzymes required for DNA replication in living cells. Explain how and why this technique only requires 1 enzyme to make lots of DNA.arrow_forwardusing the process flow of PCR describe the principle associated with the PCR technique.arrow_forwardexplain the ethical concerns for this biotechnology method (sanger sequencing)arrow_forward
- PCR is a powerful technique to screen and amplify segments of DNA for use in recombinant protein technologies. Describe in detail, the components of a PCR reaction and why they are requiredarrow_forwardPolymerase Chain Reaction (PCR) works by exposing the reaction to a series of temperature changes. In 150 words or fewer, describe 1.) the different stages of a single PCR cycle, 2.) how many cycles of PCR your reactions will undergo, and 3.) why multiple cycles are necessary.arrow_forwardBriefly present experimental and practical benefits of using PCR in DNA cloning process. Give some examples of clinical applications.arrow_forward
- Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning