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Biochemistry

9th Edition
Mary K. Campbell + 2 others
ISBN: 9781305961135

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BuyFindarrow_forward

Biochemistry

9th Edition
Mary K. Campbell + 2 others
ISBN: 9781305961135
Textbook Problem

RECALL What is blue/white screening? What is the key feature of a plasmid that is used for it?

Interpretation Introduction

Interpretation: The blue/white screening and the key feature of a plasmid, which is used for it, are to be determined.

Concept introduction:

Introduction of DNA into the genome of virus and bacteria is possible. It is known as insert.

Usually, insert is tagged to a plasmid, which is a small circular DNA present in bacteria.

The insert grows and makes multiple copies by the process of cloning.

Explanation

Cloning is the process in which identical copies of the DNA are made. For cloning a piece of DNA, first, a fragment with desired genes is identified. Then, the fragment is tagged to a plasmid, which is a small circular DNA present in bacteria. Then, this vector is introduced in the host cell known as transformation. Transformed host cells are selected and allowed to multiply to show the expression of the DNA in the host cell.

The method used to test the uptake of a plasmid with a DNA insert is done at two stages: first, when the plasmid is taken by the bacterial cell, and second, when the target DNA is taken by plasmids.

Usually, plasmids have two antibiotic resistant genes, such as for ampicillin, tetracycline, or chloramphenicol...

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