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Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant sickle cell anemia allele of beta globin. This mutation destroys an Mst II restriction site normally present in the beta globin gene. This difference between the normal allele and the mutant allele can be detected with Southern blotting. Using a labeled beta globin gene as a probe, what differences would you expect to see for a Southern blot of the normal beta globin gene and the mutant sickle cell gene?

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Human Heredity: Principles and Iss...

11th Edition
Michael Cummings
Publisher: Cengage Learning
ISBN: 9781305251052

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BuyFindarrow_forward

Human Heredity: Principles and Iss...

11th Edition
Michael Cummings
Publisher: Cengage Learning
ISBN: 9781305251052
Chapter 13, Problem 20QP
Textbook Problem
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Analyzing Cloned Sequences

A base change (A to T) is the mutational event that created the mutant sickle cell anemia allele of beta globin. This mutation destroys an MstII restriction site normally present in the beta globin gene. This difference between the normal allele and the mutant allele can be detected with Southern blotting. Using a labeled beta globin gene as a probe, what differences would you expect to see for a Southern blot of the normal beta globin gene and the mutant sickle cell gene?

Summary Introduction

To describe: The variations that can be observed between the southern blots of mutant sickle cell gene and a Southern blot of the normal beta-globin gene.

Introduction: A DNA constitutes the genetic information of an organism. The morphological makeup of an individual is depicted by the information contained by the genetic material. Any alterations caused in the sequence of DNA will result in the mutation of an individual’s genome.

Explanation of Solution

The technique of Southern blotting is responsible for the identification of the sequences that are identical in nature across various DNA samples. When a probe is used for the normal sequence, a blot without any visible fragment will be produced. As in the gene sequence, a base change causes the sickle cell anemia. In this case, the binding of probe with the sequence that is not identical for that gene does not take place. This happens due to the chance of a base, which altered the sequence and the probe is not linked to the sequence of sickle cell anemia...

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